凝固酶阴性葡萄球菌青霉素结合蛋白的凝胶电泳分析

Gel electrophoretic analysis of penicillin-binding proteins of coagulase negative staphylococci.

作者信息

Kanda K, Hiramatsu K, Yokota T

机构信息

Department of Microbiology, Faculty of Medicine, Juntendo University, Tokyo, Japan.

出版信息

FEMS Microbiol Lett. 1990 Oct;60(1-2):51-3. doi: 10.1016/0378-1097(90)90343-o.

DOI:10.1016/0378-1097(90)90343-o

PMID:2283040

Abstract

We analyzed gel electrophoretic banding patterns of penicillin-binding proteins (PBPs) of 16 type strains of coagulase-negative staphylococci (CNS). S. epidermidis, S. haemolyticus, S. saprophyticus, S. hominis, S. xylosus, S. simulans, S. warneri, S. capitis, S. saccharolyticus, S. auricularis, S. caseolyticus, S. gallinarum, S. hycus subsp. hycus, S. cohnii, S. caprae, and S. sciuri subsp. sciuri. The PBP profile of each CNS species was found to be unique and was clearly distinguishable from those of the rest of the species. Together with the previous work of other researchers, this study substantiates the applicability of the PBP profile analysis to the identification of clinical CNS strains.

摘要

我们分析了16株凝固酶阴性葡萄球菌（CNS）标准菌株的青霉素结合蛋白（PBP）的凝胶电泳条带模式。这些菌株包括表皮葡萄球菌、溶血葡萄球菌、腐生葡萄球菌、人葡萄球菌、木糖葡萄球菌、模仿葡萄球菌、沃氏葡萄球菌、头葡萄球菌、解糖葡萄球菌、耳葡萄球菌、溶酪葡萄球菌、鸡葡萄球菌、猪葡萄球菌亚种猪葡萄球菌、科氏葡萄球菌、山羊葡萄球菌以及松鼠葡萄球菌亚种松鼠葡萄球菌。结果发现，每种CNS菌株的PBP图谱都是独特的，并且与其他菌株的图谱明显不同。结合其他研究人员之前的工作，本研究证实了PBP图谱分析在临床CNS菌株鉴定中的适用性。