Pierre J, Gutmann L, Bornet M, Bergogne-Berezin E, Williamson R
Laboratoire de Microbiologie Médicale, Université Pierre et Marie Curie, Paris, France.
J Clin Microbiol. 1990 Mar;28(3):443-6. doi: 10.1128/jcm.28.3.443-446.1990.
Analyses of total solubilized proteins and penicillin-binding proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were demonstrated to be accurate methods for the identification of coagulase-negative staphylococci. However, penicillin-binding protein profiles were found to be much clearer for the identification of these organisms to species level than was examination of the total solubilized proteins. By using the former technique, 13 reference strains were found to have species-specific penicillin-binding protein profiles, and 150 of 160 randomly collected clinical isolates were identified as belonging to eight of these species. A group of three clinical strains probably represents the recently described species Staphylococcus lugdunensis; the other seven clinical isolates belonging to five species remained unclassified.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对总可溶性蛋白和青霉素结合蛋白进行分析，被证明是鉴定凝固酶阴性葡萄球菌的准确方法。然而，发现青霉素结合蛋白图谱在将这些微生物鉴定到种水平方面比检查总可溶性蛋白要清晰得多。通过使用前一种技术，发现13株参考菌株具有种特异性的青霉素结合蛋白图谱，在随机收集的160株临床分离株中，有150株被鉴定属于其中8个种。一组3株临床菌株可能代表最近描述的路邓葡萄球菌；属于5个种的其他7株临床分离株仍未分类。
