对一例乐果中毒病例体液中乐果及其代谢物的测定。

Determination of disulfoton and its metabolites in the body fluids of a Di-Syston intoxication case.

作者信息

Yashiki M, Kojima T, Ohtani M, Chikasue F, Miyazaki T

机构信息

Department of Legal Medicine, Hiroshima University School of Medicine, Japan.

出版信息

Forensic Sci Int. 1990 Dec;48(2):145-54. doi: 10.1016/0379-0738(90)90107-a.

DOI:10.1016/0379-0738(90)90107-a

PMID:2283138

Abstract

Disulfoton and its metabolites, two sulfoxides and two sulfones, in the body fluids of a patient who had ingested Di-Syston were analyzed by FPD-GC and GC/MS. After the chemicals in the extract (Fraction 1) obtained by Extrelut column extraction were analyzed, disulfoton and sulfoxides in Fraction 1 were oxidized into sulfones. The sulfones in the extract (Fraction 2) obtained by Extrelut column extraction were analyzed and the estimated concentrations of metabolite were calculated. The concentrations of disulfoton and the sum of the metabolites in the blood collected on admission were 0.093 nmol/g (25.4 ng/g) and 4.92 nmol/g (corresponding to 1.35 micrograms/g of disulfoton), respectively. These concentrations appear to indicate a severe level of disulfoton intoxication.

摘要

采用火焰光度检测气相色谱法（FPD-GC）和气相色谱-质谱联用仪（GC/MS）对一名摄入了硫丹的患者体液中的乙拌磷及其代谢物（两种亚砜和两种砜）进行了分析。在用埃特雷卢特柱萃取法得到的提取物（馏分1）中的化学物质分析完毕后，馏分1中的乙拌磷和亚砜被氧化成了砜。对用埃特雷卢特柱萃取法得到的提取物（馏分2）中的砜进行了分析，并计算了代谢物的估计浓度。入院时采集的血液中乙拌磷及其代谢物总和的浓度分别为0.093纳摩尔/克（25.4纳克/克）和4.92纳摩尔/克（相当于1.35微克/克乙拌磷）。这些浓度似乎表明乙拌磷中毒程度严重。