Yashiki M, Kojima T, Ohtani M, Chikasue F, Miyazaki T
Department of Legal Medicine, Hiroshima University School of Medicine, Japan.
Forensic Sci Int. 1990 Dec;48(2):145-54. doi: 10.1016/0379-0738(90)90107-a.
Disulfoton and its metabolites, two sulfoxides and two sulfones, in the body fluids of a patient who had ingested Di-Syston were analyzed by FPD-GC and GC/MS. After the chemicals in the extract (Fraction 1) obtained by Extrelut column extraction were analyzed, disulfoton and sulfoxides in Fraction 1 were oxidized into sulfones. The sulfones in the extract (Fraction 2) obtained by Extrelut column extraction were analyzed and the estimated concentrations of metabolite were calculated. The concentrations of disulfoton and the sum of the metabolites in the blood collected on admission were 0.093 nmol/g (25.4 ng/g) and 4.92 nmol/g (corresponding to 1.35 micrograms/g of disulfoton), respectively. These concentrations appear to indicate a severe level of disulfoton intoxication.
采用火焰光度检测气相色谱法（FPD-GC）和气相色谱-质谱联用仪（GC/MS）对一名摄入了硫丹的患者体液中的乙拌磷及其代谢物（两种亚砜和两种砜）进行了分析。在用埃特雷卢特柱萃取法得到的提取物（馏分1）中的化学物质分析完毕后，馏分1中的乙拌磷和亚砜被氧化成了砜。对用埃特雷卢特柱萃取法得到的提取物（馏分2）中的砜进行了分析，并计算了代谢物的估计浓度。入院时采集的血液中乙拌磷及其代谢物总和的浓度分别为0.093纳摩尔/克（25.4纳克/克）和4.92纳摩尔/克（相当于1.35微克/克乙拌磷）。这些浓度似乎表明乙拌磷中毒程度严重。
