Yashiki M, Kojima T, Miyazaki T, Chikasue F, Ohtani M
Department of Legal Medicine, Hiroshima University School of Medicine, Japan.
Forensic Sci Int. 1990 Aug;47(1):21-9. doi: 10.1016/0379-0738(90)90282-4.
A simple and rapid method for analysis of free and conjugated cresols in biological fluids was developed. Prior to and following freeing of the conjugated cresols by acid hydrolysis in a sealed ampoule, free cresols were extracted by Extrelut column extraction, determined by gas chromatography, and confirmed by gas chromatography-mass spectrometry. In a non-fatal case of cresol intoxication a 46-year-old male had ingested about 100 ml of a saponated cresol soap solution. The concentrations of xylenol (2,4- and/or 2,5-dimethylphenol) and p- and m-cresol in the serum sample collected on admission were 15.8 micrograms/g, 43.3 micrograms/g and 73.8 micrograms/g, respectively. The total cresol concentration of 117 micrograms/g in the serum is within the range of fatal concentrations, and it is suspected therefore that the patient's recovery was due to adequate therapy alone.
开发了一种用于分析生物体液中游离和结合甲酚的简单快速方法。在密封安瓿中通过酸水解使结合甲酚游离之前和之后，通过Extrelut柱萃取法提取游离甲酚，用气相色谱法测定，并通过气相色谱-质谱法进行确认。在一例非致命性甲酚中毒病例中，一名46岁男性摄入了约100毫升皂化甲酚皂溶液。入院时采集的血清样本中二甲酚（2,4-和/或2,5-二甲基苯酚）以及对甲酚和间甲酚的浓度分别为15.8微克/克、43.3微克/克和73.8微克/克。血清中甲酚总浓度为117微克/克，处于致命浓度范围内，因此怀疑患者的康复仅归因于充分的治疗。
