Passos Lygia Maria Friche
Departamento de Medicina Veterinária Preventiva, Instituto Nacional em Ciência e Tecnologia--Informação Genético-Sanitária da Pecuária Brasileira, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Rev Bras Parasitol Vet. 2012 Apr-Jun;21(2):81-6. doi: 10.1590/s1984-29612012000200002.
Continuous cell lines have been established from several ixodid and argasid tick species, representing an excellent tool suitable for the isolation of pathogens and their subsequent propagation, which in turn allows the production of antigenic material for diagnostic tests, antibody and vaccine production, and also for studies on host-vector-pathogen relationships. This paper reviews the use of tick cells for culture initiation and maintenance of two obligate intracellular bacterial pathogens, Anaplasma marginale and Anaplasma phagocytophilum. These in vitro cultivation systems have been used in a wide range of studies, covering morphological ultrastructural analysis, genetics, proteomics and biological differences between strains, including genome transcriptional and protein expression approaches, enabling comparisons between host and vector cells. Thus, such systems open a new window for a better understanding of interactions between pathogens and tick cells. Last but not least, such systems contribute to the reduction in usage of animals for experimental research, as antigenic material can be produced in reasonably large quantities without the use of in vivo species-specific systems.
已经从几种硬蜱和软蜱物种中建立了连续细胞系，这是一种极好的工具，适用于病原体的分离及其后续繁殖，进而能够生产用于诊断测试、抗体和疫苗生产的抗原物质，还可用于研究宿主-媒介-病原体之间的关系。本文综述了蜱细胞在启动和维持两种专性细胞内细菌病原体——边缘无形体和嗜吞噬细胞无形体培养中的应用。这些体外培养系统已用于广泛的研究，涵盖形态超微结构分析、遗传学、蛋白质组学以及菌株之间的生物学差异，包括基因组转录和蛋白质表达方法，从而能够比较宿主细胞和媒介细胞。因此，此类系统为更好地理解病原体与蜱细胞之间的相互作用打开了一扇新窗口。最后但同样重要的是，此类系统有助于减少实验研究中动物的使用，因为无需使用体内物种特异性系统就能大量生产抗原物质。
