Lasmar Pedro Veloso Facury, Carvalho Antônio Último de, Facury Filho Elias Jorge, Bastos Camila Valgas, Ribeiro Múcio Flávio Barbosa
Departamento de Clínica da Escola de Veterinaria, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
Rev Bras Parasitol Vet. 2012 Apr-Jun;21(2):112-7. doi: 10.1590/s1984-29612012000200008.
The protective efficacy of an inactivated vaccine from Anaplasma marginale that was cultured in tick cells (IDE8) for use against bovine anaplasmosis was evaluated. Five calves (Group 1) were inoculated subcutaneously, at 21-day intervals, with three doses of vaccine containing 3 × 10(9) A. marginale initial bodies. Five control calves received saline solution alone (Group 2). Thirty-two days after the final inoculation, all the calves were challenged with approximately 3 × 10(5) erythrocytes infected with A. marginale high-virulence isolate (UFMG2). The Group 1 calves seroconverted 14 days after the second dose of vaccine. After the challenge, all the animals showed patent rickettsemia. There was no significant difference (p > 0.05) between the Group 1 and 2 calves during the incubation period, patency period or convalescence period. All the animals required treatment to prevent death. The results suggest that the inactivated vaccine from A. marginale produced in IDE8 induced seroconversion in calves, but was not effective for preventing anaplasmosis induced by the UFMG2 isolate under the conditions of this experiment.
对一种在蜱细胞（IDE8）中培养的用于预防牛无形体病的边缘无形体灭活疫苗的保护效力进行了评估。五头犊牛（第1组）每隔21天皮下接种三剂含3×10⁹个边缘无形体初始体的疫苗。五头对照犊牛仅接受生理盐水（第2组）。最后一次接种后32天，所有犊牛均用约3×10⁵个感染了边缘无形体高毒力分离株（UFMG2）的红细胞进行攻毒。第1组犊牛在第二剂疫苗接种后14天出现血清转化。攻毒后，所有动物均出现明显的立克次体血症。在潜伏期、发病期或恢复期，第1组和第2组犊牛之间无显著差异（p>0.05）。所有动物均需治疗以防止死亡。结果表明，在IDE8中生产的边缘无形体灭活疫苗可诱导犊牛血清转化，但在本实验条件下，对预防UFMG2分离株诱导的无形体病无效。
