Kaomongkolgit Ruchadaporn
Department of Oral Diagnosis, Faculty of Dentistry, Naresuan University, Phitsanulok, 65000, Thailand,
Odontology. 2013 Jul;101(2):227-32. doi: 10.1007/s10266-012-0081-2. Epub 2012 Jul 26.
The purpose of this study was to investigate the effect of alpha-mangostin on matrix metalloproteinase (MMP)-2 and MMP-9 expression in head and neck squamous cell carcinoma (HNSCC). The human HNSCC cell lines were treated with alpha-mangostin and the cytotoxicity of alpha-mangostin in HNSCC was determined using the MTS assay. To determine the effect of alpha-mangostin on the expression of MMP-2 and MMP-9 in HNSCC, gelatin zymography and RT-PCR were performed. The results showed that alpha-mangostin increased in growth inhibition of HNSCC cell lines in a concentration-dependent manner. Treatment with alpha-mangostin decreased MMP-2 and MMP-9 expression in a concentration-dependent manner in all cell lines. These findings suggested that alpha-mangostin might be a potential therapeutic agent for HNSCC.
本研究的目的是探讨α-山竹黄酮对头颈部鳞状细胞癌（HNSCC）中基质金属蛋白酶（MMP）-2和MMP-9表达的影响。用人HNSCC细胞系进行α-山竹黄酮处理，并使用MTS法测定α-山竹黄酮对HNSCC的细胞毒性。为了确定α-山竹黄酮对HNSCC中MMP-2和MMP-9表达的影响，进行了明胶酶谱分析和逆转录聚合酶链反应（RT-PCR）。结果表明，α-山竹黄酮以浓度依赖性方式增加了对HNSCC细胞系的生长抑制作用。在所有细胞系中，α-山竹黄酮处理均以浓度依赖性方式降低了MMP-2和MMP-9的表达。这些发现表明，α-山竹黄酮可能是HNSCC的一种潜在治疗药物。
