Department of Ophthalmology and Visual Science, Seoul St Mary's Hospital, The College of Medicine, The Catholic University of Korea, Yeongdeungpo-gu, Seoul, Korea.
Cornea. 2012 Oct;31(10):1165-9. doi: 10.1097/ICO.0b013e318241433b.
To determine the efficacy of the topical application of ascorbic acid for the treatment of corneal neovascularization.
Corneal neovascularization was induced in 16 rabbits with a silk suture in the corneal stroma (32 eyes). At 1 week after suturing, 15 rabbits were divided into 3 groups and were treated with topical ascorbic acid at 3 different concentrations: 10 mg/mL (group 1), 1 mg/mL (group 2), and 0.5 mg/mL (group 3). All treatments were added in the right eye twice a day. All left eyes (15 eyes) and both eyes of the 16th rabbit were used as experimental controls and a normal control, respectively. The area of corneal neovascularization was measured using light microscopy. The concentrations of vascular endothelial growth factor and matrix metalloproteinase-9 in the corneal tissue were measured.
The neovascularized area was decreased in the treated groups compared with the control group. There was a significant difference in the neovascularized areas between the control and groups 1 and 2. No significant difference was observed between the control and group 3. The concentration of vascular endothelial growth factor was significantly lower in the treated groups than in the control group, but there was no difference between the treated groups. The concentration of matrix metalloproteinase-9 showed a significant difference between the control and treated groups, but no difference between the treated groups.
Topical administration of ascorbic acid may be useful for the treatment of corneal neovascularization.
确定局部应用抗坏血酸治疗角膜新生血管的疗效。
通过角膜基质中的丝线缝合在 16 只兔子中诱导角膜新生血管（32 只眼）。缝合后 1 周，将 15 只兔子分为 3 组，并以 3 种不同浓度的局部抗坏血酸进行治疗：10mg/ml（第 1 组）、1mg/ml（第 2 组）和 0.5mg/ml（第 3 组）。所有治疗均每天在右眼添加两次。所有左眼（15 只眼）和第 16 只兔子的双眼均作为实验组对照和正常对照组。使用光学显微镜测量角膜新生血管的面积。测量角膜组织中血管内皮生长因子和基质金属蛋白酶-9 的浓度。
与对照组相比，治疗组的新生血管面积减少。对照组与第 1 组和第 2 组之间的新生血管面积有显著差异。对照组与第 3 组之间没有观察到显著差异。治疗组中血管内皮生长因子的浓度明显低于对照组，但治疗组之间没有差异。基质金属蛋白酶-9 的浓度在对照组和治疗组之间存在显著差异，但治疗组之间没有差异。
局部应用抗坏血酸可能对治疗角膜新生血管有用。
