从埃及种植的长春花（Catharanthus roseus L. Don.）中分离和鉴定抗肿瘤生物碱。
Isolation and characterization of antineoplastic alkaloids from Catharanthus roseus L. Don. cultivated in Egypt.
作者信息
Shams Khaled A, Nazif Naglaa M, Abdel Azim Nahla S, Abdel Shafeek Khaled A, El-Missiry Mostafa M, Ismail Shams I, Seif El Nasr Medhat M
机构信息
Phytochemistry Department, National Research Centre, Dokki, Giza, Egypt, 12311.
出版信息
Afr J Tradit Complement Altern Med. 2009 Mar 7;6(2):118-22. doi: 10.4314/ajtcam.v6i2.57082.
Abstract
Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed bed vacuum liquid chromatography (VLC), Charcoal column, and finally purified by centrifugally accelerated radial chromatography (Chromatotrone).
摘要
长春碱和长春新碱（抗白血病药物）通过多种色谱技术，以纯形式从在埃及种植的长春花中分离得到。开展了五种制备总生物碱的改良方法。所有分离得到的混合物均通过高效液相色谱（HPLC）和高效薄层色谱（HPTLC）分析进行评估。通过使用硅胶：氧化铝（1:1）混合床真空液相色谱（VLC）的真空液相色谱柱、活性炭柱，并最终通过离心加速径向色谱（Chromatotrone）对抗肿瘤生物碱长春碱和长春新碱进行分离。
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