Labovitiadi Olga, Lamb Andrew J, Matthews Kerr H
Institute for Health & Welfare Research, Robert Gordon University, School Hill, Aberdeen, AB10 1FR, UK.
Ther Deliv. 2012 Apr;3(4):443-55. doi: 10.4155/tde.12.27.
Lyophilized wafers have been developed as vehicles for the storage and delivery of therapeutic compounds to exuding wounds. The primary objective of this study was to incorporate a selection of antimicrobial compounds in karaya wafers and measure their efficacy against methicillin-resistant Staphylococcus aureus.
Four antimicrobial compounds, including an antibiotic, were incorporated within karaya gels and freeze-dried to a shaped-disc form suitable for in vitro testing against methicillin-resistant S. aureus. Disc diffusion and Franz diffusion methods were used to quantify the utility and efficacy of these 'antimicrobial wafers'. Flow rheology and scanning electron microscopy were also used to aid gel and wafer characterization.
Lyophilized wafers swelled in simulated wound fluid and released the contained compounds with mixed effect. Povidine-iodine and chlorhexidine were most effective in protein-free buffer while the action of neomycin sulfate was enhanced by the presence of bovine serum albumin. Silver sulfadiazine was the least effective overall.
冻干薄片已被开发用作将治疗性化合物储存和递送至渗出性伤口的载体。本研究的主要目的是在卡拉亚薄片中加入一系列抗菌化合物，并测定它们对耐甲氧西林金黄色葡萄球菌的疗效。
将四种抗菌化合物（包括一种抗生素）加入卡拉亚凝胶中，并冷冻干燥成适合体外测试耐甲氧西林金黄色葡萄球菌的圆盘状。采用纸片扩散法和弗朗兹扩散法来量化这些“抗菌薄片”的效用和疗效。还使用了流变学和扫描电子显微镜来辅助凝胶和薄片的表征。
冻干薄片在模拟伤口液中膨胀，并以混合效应释放所含化合物。聚维酮碘和洗必泰在无蛋白缓冲液中最有效，而硫酸新霉素的作用因牛血清白蛋白的存在而增强。磺胺嘧啶银总体效果最差。
