Department of Molecular Genetics and Biotechnology, Institute of Cell Biology, National Academy of Sciences of Ukraine, 79005 Lviv, Ukraine.
Int J Biochem Cell Biol. 2012 Nov;44(11):1906-18. doi: 10.1016/j.biocel.2012.07.017. Epub 2012 Jul 23.
In the yeast Saccharomyces cerevisiae, the one-at-a-time deletions of either the high-affinity glucose sensor gene SNF3 or the low-affinity glucose sensor gene RGT2 only slightly reduced pexophagy; however, deleting both genes greatly reduced pexophagy, evincing interaction beyond the sum of the additive effects, as recently shown. The present study identifies the only ScSNF3/RGT2 ortholog in the methylotrophic yeast Pichia pastoris (designated as PpGSS1, from GlucoSe Sensor) and describes its roles in autophagic pathways (non-selective and selective). GSS1 knock-out strain has been constructed. The experiments support the hypothesis that Gss1 plays an important role in autophagic degradation of peroxisomes and glucose catabolite repression in P. pastoris.
在酵母酿酒酵母中，高亲和力葡萄糖传感器基因 SNF3 或低亲和力葡萄糖传感器基因 RGT2 的逐个缺失仅略微降低了过氧化物酶体自噬；然而，删除这两个基因大大降低了过氧化物酶体自噬，表明存在超出加性效应总和的相互作用，正如最近所表明的那样。本研究鉴定了甲基营养酵母巴斯德毕赤酵母中唯一的 ScSNF3/RGT2 直系同源物（命名为 PpGSS1，来自 GlucoSe Sensor），并描述了其在自噬途径（非选择性和选择性）中的作用。已经构建了 GSS1 敲除株。实验支持了这样的假设，即 Gss1 在巴斯德毕赤酵母过氧化物酶体的自噬降解和葡萄糖分解代谢物阻遏中发挥重要作用。
