Moscoso H, Kiefer C R, Shyamala M, von Dohlen T, Garver F A
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.
J Clin Lab Anal. 1990;4(6):437-42. doi: 10.1002/jcla.1860040609.
We have developed a monoclonal antibody-based enzyme immunoassay and a solid-phase radioimmunoassay for human myoglobin. Both assays are based on competition for the monoclonal antibody between the free myoglobin present in the standards or serum samples and the myoglobin coated to the wells of microtiter plates. Consequently, the absorbance at 630 nm and the radioactivity are inversely related to the concentrations of free myoglobin. The sensitivity of both assays was 10 micrograms/L with linearity up to 1,000 micrograms/L. There was no interference with other serum proteins, as judged from analysis of specimens with high concentrations of lactate dehydrogenase, creatine kinase, or hemoglobin. The average serum myoglobin concentration in 30 normal individuals was 67 micrograms/L. Five patients with cardiac arrhythmias had normal values (average, 63 micrograms/L) while four patients with myocardial infarction had abnormally high concentrations of myoglobin (300-1,000 + micrograms/L). In a typical case of myocardial infarction, serum myoglobin rose 21 hr earlier and peaked 12 hr earlier than creatine kinase and its cardiac isoenzyme. These rapid immunoassays appear to be useful for the early detection of increased serum myoglobin indicative of myocardial infarction.
我们已开发出一种基于单克隆抗体的酶免疫测定法和一种用于检测人肌红蛋白的固相放射免疫测定法。两种测定法均基于标准品或血清样本中游离肌红蛋白与包被在微量滴定板孔中的肌红蛋白之间对单克隆抗体的竞争。因此，630nm处的吸光度和放射性与游离肌红蛋白的浓度呈负相关。两种测定法的灵敏度均为10微克/升，线性范围高达1000微克/升。通过对高浓度乳酸脱氢酶、肌酸激酶或血红蛋白标本的分析判断，未发现其他血清蛋白的干扰。30名正常个体的血清肌红蛋白平均浓度为67微克/升。5名心律失常患者的值正常（平均63微克/升），而4名心肌梗死患者的肌红蛋白浓度异常高（300 - 1000 +微克/升）。在典型的心肌梗死病例中，血清肌红蛋白比肌酸激酶及其心肌同工酶提前21小时升高，提前12小时达到峰值。这些快速免疫测定法似乎有助于早期检测出提示心肌梗死的血清肌红蛋白升高。
