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Chemiluminescent immunosorbent assay of serum myoglobin based on the luminol reaction.

作者信息

Olsson T, Bergström K, Thore A

出版信息

Clin Chim Acta. 1984 Mar 27;138(1):31-40. doi: 10.1016/0009-8981(84)90351-6.

DOI:10.1016/0009-8981(84)90351-6
PMID:6370508
Abstract

A simple and sensitive method for the determination of serum myoglobin is described. Myoglobin was determined in the range of 10-500 micrograms/1 by the chemiluminescent luminol reaction after adsorption to anti-myoglobin IgG onto a solid phase. Only 50 microliter of serum has to be used and the luminescent immunoassay (LIA) has a better sensitivity and a wider linear range than the conventional radioimmunoassay (RIA). However, in its present form LIA has a higher imprecision than RIA. In clinical specimens the correlation between the two methods was excellent.

摘要

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