Hansen K N, Nørgaard-Pedersen B
Scand J Clin Lab Invest. 1979 Oct;39(6):525-31. doi: 10.1080/00365517909108830.
A radioimmunoassay for quantitation of serum myoglobin in healthy individuals and patients with different diseases is described. Purified myoglobin was labelled by an 125I-labelled ester (N-succinimidyl 3-(-4 hydroxy, 5-[125I]iodophenyl) propionate), a commercially available antiserum was used, and the antigen-antibody complex was precipitated with polyethylene glycol 6000. The rapid assay can be performed within 1 h at 37 degrees C with a detection limit of 45 micrograms/l. Prolonged incubation at 4 degrees C for 18 or 72 h gives a detection limit of 6 and 2 micrograms/l, respectively. The mean coefficient of variation of the routine assay was 11%. In healthy human subjects a significant difference in mean serum myoglobin concentration was found between 43 women (34 +/- 17 micrograms/l) and 51 mean 47 +/- 15 micrograms/l). In twenty patients admitted to hospital with the clinical diagnosis acute myocardial infarction, the serum myoglobin concentration profiles were in close agreement with the final diagnosis. In three patients with myocardial infarction serum samples were taken every 2 h after the acute episode, and serum myoglobin levels were compared with the levels of creatine kinase, lactate dehydrogenase, aspartate aminotransferase and creatine kinase isoenzyme-MB.
本文描述了一种用于定量检测健康个体和不同疾病患者血清肌红蛋白的放射免疫分析法。纯化的肌红蛋白用125I标记的酯（N-琥珀酰亚胺基3-（-4-羟基，5-[125I]碘苯基）丙酸酯）进行标记，使用市售抗血清，并用聚乙二醇6000沉淀抗原-抗体复合物。该快速检测法可在37℃下1小时内完成，检测限为45微克/升。在4℃下延长孵育18或72小时，检测限分别为6和2微克/升。常规检测的平均变异系数为11%。在健康人体受试者中，发现43名女性（34±17微克/升）和51名男性（平均47±15微克/升）的平均血清肌红蛋白浓度存在显著差异。在20例临床诊断为急性心肌梗死入院的患者中，血清肌红蛋白浓度变化与最终诊断密切相关。在3例心肌梗死患者急性发作后每2小时采集血清样本，并将血清肌红蛋白水平与肌酸激酶、乳酸脱氢酶、天冬氨酸转氨酶和肌酸激酶同工酶-MB的水平进行比较。
