Griffith C D, Ellis I O, Bell J, Burns K, Blamey R W
University Department of Surgery and Pathology, City Hospital, Nottingham, UK.
J R Coll Surg Edinb. 1990 Oct;35(5):289-92.
Lymphocytic infiltration of breast cancer is often associated with a favourable prognosis. Seventy-seven women with operable breast cancer were followed for a minimum of 3 years. Tumours were frozen and sectioned by cryostat before staining with monoclonal antibodies using an immunoperoxide technique for total lymphocytes, helper/inducer, suppressor/cytotoxic, natural killer and B subsets. Lymphocyte density was assessed by light microscopy at x400 and divided at the 50th percentile to less than 30 and greater than or equal to 30 cells per high power field to give scanty and dense lymphocytic infiltration. The helper/suppressor lymphocyte ratio was greater than 1 in 45 patients but without improvement in survival or cancer recurrence. Natural killer and B lymphocytes were rarely seen in tumour sections. Poorly differentiated tumours excited a more marked lymphocytic infiltration (P less than 0.01). Neither the density of lymphocyte infiltration nor the ratio of helper to suppressor lymphocytes correlated with improved short-term survival or recurrence. These data suggest that the immune defence is ineffective in preventing spread from the primary tumour in breast cancer patients.
乳腺癌的淋巴细胞浸润通常与良好的预后相关。77例可手术乳腺癌患者被随访至少3年。肿瘤被冷冻并用低温恒温器切片，然后使用免疫过氧化物技术用单克隆抗体对总淋巴细胞、辅助/诱导性、抑制/细胞毒性、自然杀伤细胞和B细胞亚群进行染色。通过光学显微镜在400倍放大倍数下评估淋巴细胞密度，并在第50百分位数处划分为每高倍视野少于30个细胞和大于或等于30个细胞，分别表示淋巴细胞浸润稀少和密集。45例患者的辅助/抑制淋巴细胞比值大于1，但生存率或癌症复发情况并无改善。在肿瘤切片中很少见到自然杀伤细胞和B淋巴细胞。低分化肿瘤引发更明显的淋巴细胞浸润（P小于0.01）。淋巴细胞浸润密度和辅助与抑制淋巴细胞的比值均与短期生存率或复发改善无关。这些数据表明，免疫防御在预防乳腺癌患者原发肿瘤的扩散方面无效。
