Department of Family Medicine, Medical University of Warsaw, Warsaw, Poland.
Adv Exp Med Biol. 2013;756:213-21. doi: 10.1007/978-94-007-4549-0_27.
The role of angiogenesis in the pathogenesis of interstitial lung diseases (ILD) is unknown. Angiotensin-converting enzyme (ACE) is a marker of sarcoidosis activity and may modulate angiogenesis. The aim of this study was to examine the relationship between ACE activity in ILD patients' sera and their effect on microvessels formation in an in vivo model of leukocyte-induced angiogenesis. The study population consisted of 77 sarcoidosis patients, 22 idiopathic pulmonary fibrosis patients, 16 bird fanciers lung patients, eight silicosis patients and 14 healthy donors. Serum ACE activity was assayed by spectrophotometric method. As an angiogenic test, a leukocyte-induced angiogenesis assay in an animal model was used. Sera from interstitial lung disease patients significantly stimulated angiogenic activity of mononuclear cells compared with healthy donors (p < 0.001). The highest ACE serum activity was measured in sera from the silicosis patients, and lowest in sera from the sarcoidosis and IPF patients. A significantly lower serum ACE activity was detected in the bird fanciers lung patients. Serum angiogenic activity of ILD patients measured by angiogenesis index negatively correlated with ACE serum activity (r = ;-0.52; p < 0.01). This correlation was highest in the sarcoidosis group (r = -0.6; p < ). Sera from ILD patient constitute the source of factors modulating angiogenesis.
血管生成在间质性肺疾病（ILD）发病机制中的作用尚不清楚。血管紧张素转换酶（ACE）是结节病活动的标志物，可能调节血管生成。本研究旨在研究ILD 患者血清中 ACE 活性与白细胞诱导的血管生成体内模型中微血管形成的关系。研究人群包括 77 例结节病患者、22 例特发性肺纤维化患者、16 例鸟爱好者肺患者、8 例矽肺患者和 14 例健康供体。通过分光光度法测定血清 ACE 活性。作为血管生成试验，使用动物模型中的白细胞诱导的血管生成试验。与健康供体相比，ILD 患者的血清显着刺激单核细胞的血管生成活性（p <0.001）。矽肺患者的血清 ACE 活性最高，结节病和特发性肺纤维化患者的血清 ACE 活性最低。鸟爱好者肺患者的血清 ACE 活性显着降低。通过血管生成指数测量的 ILD 患者的血清血管生成活性与 ACE 血清活性呈负相关（r = ；-0.52；p <0.01）。在结节病组中，这种相关性最高（r = -0.6；p <0.01）。ILD 患者的血清构成调节血管生成的因素的来源。
