Department of Pediatric and Endocrinology, Warsaw Medical University, Warsaw, Poland.
Adv Exp Med Biol. 2013;756:255-62. doi: 10.1007/978-94-007-4549-0_32.
Fat mass content, fat distribution, and fat-mass and obesity associated (FTO) gene have been reported among a broad spectrum of genetic variation connected with body weight. The aim of our study was to investigate whether the T/A rs9939609 polymorphism of the FTO gene may influence obesity and metabolic indices in children. A 160 children were examined (136 obese and 24 non-obese). The anthropometric measurements and calculations included: height, weight, waist and hip circumference, sum of the thickness of 3 and 10 skin folds, % of fat content, % FAT- BIA , % LBM-BIA. BMI, SDS of BMI, WHR, and WHtR. Fasting plasma total cholesterol (TC), HDL and LDL-cholesterol, triglycerides (TG), oral glucose tolerance test (OGTT), and HOMA-IR were analyzed and the blood pressure were measured. The rs9939609 polymorphism of FTO gene was genotyped by allele-specific real-time polymerase chain- reaction (RT-PCR). We found that the mean concentrations of TC, TG, LDLC, and HOMA-IR were significantly higher, and HDL was lower in the obese than in non-obese children. The presence of TT, but not AA alleles, related to the percentage of fat content, BMI, and z-score of BMI. None of the other anthropometric indices did differ between the children with gene polymorphism and wild homozygous. In conclusion, rs9939609 polymorphism in the fat-mass and obesity-associated gene is associated with BMI and the percent of fat content in children.
脂肪含量、脂肪分布以及与体重相关的脂肪质量和肥胖相关（FTO）基因在广泛的与体重相关的遗传变异中都有报道。我们的研究目的是调查 FTO 基因的 T/A rs9939609 多态性是否会影响儿童肥胖和代谢指标。我们检查了 160 名儿童（136 名肥胖儿童和 24 名非肥胖儿童）。人体测量和计算包括：身高、体重、腰围和臀围、3 个和 10 个皮肤褶皱厚度的总和、脂肪含量百分比、%FAT-BIA、%LBM-BIA、BMI、BMI 的 SDS、腰臀比（WHR）和腰高比（WHtR）。分析了空腹血浆总胆固醇（TC）、高密度脂蛋白（HDL）和低密度脂蛋白胆固醇（LDL-C）、甘油三酯（TG）、口服葡萄糖耐量试验（OGTT）和 HOMA-IR，并测量了血压。通过等位基因特异性实时聚合酶链反应（RT-PCR）对 FTO 基因的 rs9939609 多态性进行了基因分型。我们发现，肥胖儿童的 TC、TG、LDLC 和 HOMA-IR 浓度显著升高，而 HDL 浓度较低。与非肥胖儿童相比，TT 等位基因的存在而非 AA 等位基因的存在与脂肪含量百分比、BMI 和 BMI 的 z 评分有关。具有基因多态性和野生纯合子的儿童之间的其他人体测量指标没有差异。总之，FTO 基因的 rs9939609 多态性与儿童 BMI 和脂肪含量百分比有关。
