Molecular Imaging Center, Department of Chemistry, University of Turin, Italy.
Magn Reson Med. 2013 Jun;69(6):1703-11. doi: 10.1002/mrm.24411. Epub 2012 Jul 26.
Conventional T1- or T2-MRI contrast agents do not allow to track the distribution of different cell populations simultaneously because the effects of relaxation enhancers are additive. Herein, it is shown that paramagnetic chemical exchange saturation transfer agents offer the opportunity to visualize different cell populations in vitro and in vivo by 1H-MRI. Yb- and Eu-HPDO3A complexes have been used to label murine macrophages (J774.A1) and melanoma cells (B16-F10), respectively. By selective irradiation of the highly-shifted OH resonances of the two chemical exchange saturation transfer agents, it has been shown that tracking of the two cell types is possible. These PARAmagnetic Chemical Exchange Saturation Transfer agents have a tremendous potential for clinical translation as they share the same stability and in vivo pharmacokinetic properties of Gd-HPDO3A (ProHance®), which is a widely used clinically approved MRI agent.
传统的 T1 或 T2-MRI 对比剂不能同时跟踪不同细胞群体的分布，因为弛豫增强剂的作用是累加的。本文表明，顺磁化学交换饱和转移剂通过 1H-MRI 提供了可视化体外和体内不同细胞群体的机会。Yb 和 Eu-HPDO3A 配合物分别用于标记鼠巨噬细胞（J774.A1）和黑色素瘤细胞（B16-F10）。通过选择性辐照两个化学交换饱和转移剂的高位移 OH 共振，可以证明两种细胞类型的跟踪是可能的。这些顺磁化学交换饱和转移剂具有巨大的临床转化潜力，因为它们与 Gd-HPDO3A（ProHance®）具有相同的稳定性和体内药代动力学特性，后者是一种广泛应用于临床的批准 MRI 造影剂。
