Jurgielewicz Piotr, Harmsen Stefan, Wei Elizabeth, Bachmann Michael H, Ting Richard, Aras Omer
Pennsylvania State University College of Medicine, Hershey, PA, 17033, USA.
Department of Pediatrics, Stanford University, Stanford, CA, 94305, USA.
Cell Mol Life Sci. 2017 Dec;74(24):4455-4469. doi: 10.1007/s00018-017-2584-z. Epub 2017 Jul 3.
Cell fate is a concept used to describe the differentiation and development of a cell in its organismal context over time. It is important in the field of regenerative medicine, where stem cell therapy holds much promise but is limited by our ability to assess its efficacy, which is mainly due to the inability to monitor what happens to the cells upon engraftment to the damaged tissue. Currently, several imaging modalities can be used to track cells in the clinical setting; however, they do not satisfy many of the criteria necessary to accurately assess several aspects of cell fate. In recent years, reporter genes have become a popular option for tracking transplanted cells, via various imaging modalities in small mammalian animal models. This review article examines the reporter gene strategies used in imaging modalities such as MRI, SPECT/PET, Optoacoustic and Bioluminescence Imaging. Strengths and limitations of the use of reporter genes in each modality are discussed.
细胞命运是一个用于描述细胞在其机体环境中随时间推移而发生分化和发育的概念。它在再生医学领域非常重要，在该领域中，干细胞疗法前景广阔，但受到我们评估其疗效能力的限制，这主要是由于无法监测细胞植入受损组织后会发生什么。目前，有几种成像方式可用于在临床环境中追踪细胞；然而，它们并不满足准确评估细胞命运多个方面所需的许多标准。近年来，报告基因已成为通过小型哺乳动物动物模型中的各种成像方式追踪移植细胞的热门选择。这篇综述文章探讨了在MRI、SPECT/PET、光声成像和生物发光成像等成像方式中使用的报告基因策略。讨论了在每种成像方式中使用报告基因的优点和局限性。
