Electrical and Computer Engineering, Photonics Center, Boston University, Boston, Massachusetts 02215, United States.
Nano Lett. 2012 Sep 12;12(9):4817-22. doi: 10.1021/nl302266u. Epub 2012 Aug 23.
In this paper, we demonstrate a novel method for high throughput patterning of bioprobes with nanoscale features on biocompatible polymer substrate. Our technique, based on nanostencil lithography, employs high resolution and robust masks integrated with array of reservoirs. We show that the smallest pattern size can reach down to 100 nm. We also show that different types of biomolecules can be patterned on the same substrate simultaneously. Furthermore, the stencil can be reused multiple times to generate a series of identical patterns at low cost. Finally, we demonstrate that biomolecules can be covalently patterned on the surface while retaining their biofunctionalities. By offering the flexibility on the nanopattern design and enabling the reusability of the stencil, our approach significantly simplifies the bionanopatterning process and therefore could have profound implications in diverse biological and medical applications.
在本文中，我们展示了一种在生物相容性聚合物基底上进行具有纳米级特征的生物探针高通量图案化的新方法。我们的技术基于纳米模板光刻，采用高分辨率和稳健的掩模与阵列储液器集成。我们表明，最小的图案尺寸可以达到 100nm。我们还表明，可以在同一基底上同时对不同类型的生物分子进行图案化。此外，该模板可以重复使用多次，以低成本生成一系列相同的图案。最后，我们证明可以在保留生物分子生物功能性的情况下将其共价图案化在表面上。通过提供纳米图案设计的灵活性并实现模板的可重复使用，我们的方法显著简化了生物纳米图案化过程，因此可能在各种生物和医学应用中具有深远的意义。
