Institut Méditerranéen d'Océanologie, équipe Microbiologie Environnementale et Biotechnologie, Aix-Marseille Université, Polytech Marseille, Marseille Cedex 09, France.
Curr Microbiol. 2012 Nov;65(5):561-7. doi: 10.1007/s00284-012-0195-3. Epub 2012 Jul 28.
A microbial enrichment culture from brackish sediments was able to grow on octadec-1-ene (an unsaturated aliphatic hydrocarbon) as sole source of carbon and energy, under methanogenic conditions. Octadecene degradation is stopped either when bromoethanesulfonic acid, a selective inhibitor of methanogenesis is introduced, or when hydrogen is introduced. In the presence of bromoethanesulfonic acid, the degradation is restored by the addition of a hydrogenotrophic sulfate-reducing microorganism with sulfate. Results of molecular biodiversity, which revealed the presence of bacteria as well as of acetoclastic and hydrogenotrophic methanogens, are consistent with a syntrophic degradation involving Bacteria and Archaea. This is the first demonstration of syntrophic alkene degradation by microbial communities, showing that syntrophy is more widespread than we could have thought so far. These results highlight the need for a better understanding of microbial interactions and their role in the organic-matter degradation in polluted environments.
从咸水沉积物中富集培养的微生物能够在产甲烷条件下，以十八碳-1-烯（一种不饱和脂肪烃）作为唯一的碳源和能源进行生长。当引入溴乙磺酸钠（一种选择性的产甲烷抑制剂）或氢气时，十八碳烯的降解就会停止。在溴乙磺酸钠存在的情况下，通过添加一种具有硫酸盐的氢营养硫酸盐还原微生物，可以恢复降解。分子生物多样性的结果表明，存在细菌以及乙酸营养型和氢营养型产甲烷菌，这与涉及细菌和古菌的共代谢降解一致。这是微生物群落共代谢烯烃降解的首次证明，表明共代谢比我们迄今为止想象的更为普遍。这些结果强调了需要更好地了解微生物相互作用及其在污染环境中有机物质降解中的作用。
