Chang Wook, Um Youngsoon, Hoffman Brendan, Pulliam Holoman Tracey R
Department of Chemical Engineering, University of Maryland, College Park, Maryland 20742, USA.
Biotechnol Prog. 2005 May-Jun;21(3):682-8. doi: 10.1021/bp049579l.
Previous research demonstrated that methanogenic cultures enriched from Baltimore Harbor (Baltimore, MD) sediments were able to degrade naphthalene and phenanthrene. In this report, the degradation activity was maintained through a sequential transfer without adding additional sediments and the established polycyclic aromatic hydrocarbon (PAH)-degrading methanogenic communities were characterized via comparative sequence analysis of clone libraries of 16S rRNA genes amplified using bacteria-specific and Archaea-specific primers. The phylogenetic analysis indicated that the addition of PAHs clearly shifted the structure of the methanogenic community and resulted in an increase in populations of species previously found in other hydrocarbon-degrading communities. Of particular interest is the fact that the dominant microbial population of the naphthalene cultures was different from that of the phenanthrene cultures, suggesting that different species are involved in the degradation. Finally, this information may lead to the identification and isolation of methanogenic populations that can degrade PAHs.
先前的研究表明，从巴尔的摩港（马里兰州巴尔的摩）沉积物中富集的产甲烷培养物能够降解萘和菲。在本报告中，通过连续传代维持了降解活性，无需添加额外的沉积物，并且通过对使用细菌特异性和古菌特异性引物扩增的16S rRNA基因克隆文库进行比较序列分析，对已建立的多环芳烃（PAH）降解产甲烷群落进行了表征。系统发育分析表明，添加多环芳烃明显改变了产甲烷群落的结构，并导致先前在其他烃降解群落中发现的物种数量增加。特别值得关注的是，萘培养物的优势微生物种群与菲培养物的不同，这表明不同的物种参与了降解过程。最后，这些信息可能有助于识别和分离能够降解多环芳烃的产甲烷种群。
