Chang Wook, Um Youngsoon, Holoman Tracey R Pulliam
Department of Chemical Engineering, University of Maryland, College Park, Maryland 20742, USA.
Biotechnol Lett. 2006 Mar;28(6):425-30. doi: 10.1007/s10529-005-6073-3.
Baltimore Harbor (Baltimore, MD) sediments were utilized to initiate anaerobic enrichment cultures with polycyclic aromatic hydrocarbons (PAHs) in the absence of supplementary electron acceptors. Cultures amended with naphthalene and phenanthrene exhibited sustained, transferable degradation of the PAHs. Bromoethanesulfonic acid, a selective inhibitor of methanogenesis, inhibited the degradation of 200 microM: naphthalene and phenanthrene; molecular characterization based on 16S rRNA sequences confirmed that methanogenic Archaea were eliminated, thus providing evidence that methanogenesis is involved in the degradation pathway.
巴尔的摩港（马里兰州巴尔的摩市）的沉积物被用于在没有补充电子受体的情况下，以多环芳烃（PAHs）启动厌氧富集培养。用萘和菲改良的培养物表现出对PAHs的持续、可转移降解。溴乙烷磺酸，一种产甲烷作用的选择性抑制剂，抑制了200微摩尔萘和菲的降解；基于16S rRNA序列的分子表征证实产甲烷古菌被消除，从而提供了产甲烷作用参与降解途径的证据。
