Starnes Taylor W, Huttenlocher Anna
Microbiology Doctoral Training Program and Medical Scientist Training Program, University of Wisconsin-Madison, Madison, WI 53706, USA.
Adv Hematol. 2012;2012:398640. doi: 10.1155/2012/398640. Epub 2012 Jul 12.
The precise control of neutrophil-mediated inflammation is critical for both host defense and the prevention of immunopathology. In vivo imaging studies in zebrafish, and more recently in mice, have made the novel observation that neutrophils leave a site of inflammation through a process called neutrophil reverse migration. The application of advanced imaging techniques to the genetically tractable, optically transparent zebrafish larvae was critical for these advances. Still, the mechanisms underlying neutrophil reverse migration and its effects on the resolution or priming of immune responses remain unclear. Here, we review the current knowledge of neutrophil reverse migration, its potential roles in host immunity, and the live imaging tools that make zebrafish a valuable model for increasing our knowledge of neutrophil behavior in vivo.
中性粒细胞介导的炎症反应的精确控制对于宿主防御和免疫病理学预防都至关重要。斑马鱼以及最近在小鼠体内进行的成像研究有了新发现，即中性粒细胞通过一种称为中性粒细胞反向迁移的过程离开炎症部位。将先进的成像技术应用于具有遗传易处理性、光学透明的斑马鱼幼虫对于这些进展至关重要。然而，中性粒细胞反向迁移的潜在机制及其对免疫反应消退或启动的影响仍不清楚。在这里，我们综述了目前关于中性粒细胞反向迁移的知识、其在宿主免疫中的潜在作用，以及使斑马鱼成为增加我们对中性粒细胞体内行为认识的宝贵模型的活体成像工具。
