Jeleniewicz K, Brzeski Z, Szczepaniak S, Markowska M
Katedry i Zakładu Chemii Toksykologicznej AM w Lublinie.
Przegl Lek. 1990;47(8):581-3.
The purpose of the study was the evaluation of blood serum concentration of selected free amino acids of collagen (arginine, glycine, glutamic acid with threonine, leucine with isoleucine and alanine) in patients with diagnosed collagen pneumoconiosis caused by industrial dust containing free silica. The group studied consisted of 17 men aged 41 to 60 years with radiologically diagnosed pneumoconiosis as p, q, or r type. The exposure time to silica dust was equal to 17 years on average. The control group consisted of 21 healthy subject, men aged 16 to 59 years. The serum amino acids were isolated using paper chromatography (the cylindrical technique) and quantitative measurements were performed by means of evaluation of absorption of colorful eluates of DYDA complex with cadmium ions. The results obtained were statistically evaluated using the Student t test at 95% probability level. The statistical analysis of results showed important individual variability of amino acid determined and individual variability coefficients were in range 28.7-39.8%. The significant increase of the glycine and of glutaminic acid with threonine levels (36.4% and 62.2%, respectively) and significant decrease in the level of leucine with isoleucine (16.5%) has been stated in patients if compare with the control group. In patients with collagen pneumoconiosis the levels of arginine or alanine remained within the normal range.
本研究的目的是评估因含有游离二氧化硅的工业粉尘导致胶原性尘肺的患者血清中特定的游离胶原氨基酸（精氨酸、甘氨酸、谷氨酸与苏氨酸、亮氨酸与异亮氨酸以及丙氨酸）的浓度。研究组由17名年龄在41至60岁之间、经放射学诊断为p型、q型或r型尘肺的男性组成。二氧化硅粉尘的平均接触时间为17年。对照组由21名年龄在16至59岁之间的健康男性受试者组成。血清氨基酸采用纸色谱法（柱形技术）分离，并通过评估镉离子与DYDA复合物的有色洗脱液的吸收进行定量测量。所获结果在95%概率水平下使用学生t检验进行统计学评估。结果的统计分析显示所测定氨基酸存在显著的个体差异，个体变异系数在28.7 - 39.8%范围内。与对照组相比，患者组中甘氨酸以及谷氨酸与苏氨酸水平显著升高（分别为36.4%和62.2%），亮氨酸与异亮氨酸水平显著降低（16.5%）。在胶原性尘肺患者中，精氨酸或丙氨酸水平保持在正常范围内。
