Sapierzyński R, Dolka I, Fabisiak M
Department of Pathology and Veterinary Diagnostic, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159c, 02-766 Warsaw, Poland.
Pol J Vet Sci. 2012;15(2):247-52. doi: 10.2478/v10181-011-0141-5.
The aim of this study was to compare the concordance of immunophenotype established with routine cytopathology (slides stained with Giemsa solution) and immunocytochemistry according to CD3 and CD79 alpha immunoreactivity. The study was performed on cytological samples of 70 canine lymphomas subtyped on the basis of the updated Kiel classification system. Additionally, cytologic samples were examined immunocytochemically for the CD3 and CD79 alpha antigens presence and thus immunophenotype of neoplastic growth was confirmed. The cytopathological and immunocytochemical diagnoses were then compared; in order to measure the concordance between immunocytochemistry (IC) result and Giemsa stain result of the same sample the Cohen's kappa coefficient was calculated. On the basis of the results of immunocytochemistry of 70 cases of canine lymphoma examined 42 were recognized as B cell lymphoma and 28 as T cell lymphoma. Full accordance between the results of routine cytopathology and IC was obtained in 63 out of 70 examined dogs (90% of cases). It can be concluded that cytopathological examination of Giemsa stained smears is helpful in determining the lymphoma cells immunophenotype. Additionally, it seems that combination of routine cytopathology and immunocytochemistry in cases of canine lymphomas allows to obtain the precise diagnosis in 90% of cases, and allows to receive most important information that is necessary for planning of appropriate therapy and to determine prognosis. Finally, this routine procedure allowed to eliminate the need of collection of tissue samples during surgery or core-biopsy and thus time, cost and patient discomfort related to more complex and invasive medical procedures can be easily reduced.
本研究的目的是根据CD3和CD79α免疫反应性，比较常规细胞病理学（吉姆萨溶液染色玻片）和免疫细胞化学所确立的免疫表型的一致性。该研究在70例根据更新的基尔分类系统进行亚型分类的犬淋巴瘤细胞学样本上进行。此外，对细胞学样本进行免疫细胞化学检查，以检测CD3和CD79α抗原的存在，从而确认肿瘤生长的免疫表型。然后比较细胞病理学和免疫细胞化学诊断结果；为了测量同一样本免疫细胞化学（IC）结果与吉姆萨染色结果之间的一致性，计算了科恩kappa系数。根据70例犬淋巴瘤的免疫细胞化学结果，42例被诊断为B细胞淋巴瘤，28例为T细胞淋巴瘤。在70只接受检查的犬中，有63只（90%的病例）常规细胞病理学结果与IC结果完全一致。可以得出结论，吉姆萨染色涂片的细胞病理学检查有助于确定淋巴瘤细胞的免疫表型。此外，对于犬淋巴瘤病例，常规细胞病理学和免疫细胞化学相结合似乎能在90%的病例中获得准确诊断，并能获取规划适当治疗和确定预后所需的最重要信息。最后，这种常规程序无需在手术或芯针活检时采集组织样本，因此可以轻松减少与更复杂和侵入性医疗程序相关的时间、成本和患者不适。
