Department of Pediatrics, Obstetrics and Reproductive Medicine, University of Siena, Italy.
Am J Reprod Immunol. 2012 Nov;68(5):392-9. doi: 10.1111/j.1600-0897.2012.01180.x. Epub 2012 Jul 30.
LABELED PROBLEM: To investigate regulation of activin A and related molecules in placenta/fetal membranes from preterm premature rupture of membranes (pPROM) associated with acute chorioamnionitis (ACA).
Tissues were obtained from women with spontaneous preterm deliveries (PTD), pPROM without ACA, pPROM with ACA. Activin A, follistatin, and nodal and cripto mRNA were measured by RT-PCR.
Activin A mRNA was up-regulated in tissues from pPROM, in presence or absence of HCA, respect to PTD and in pPROM with ACA respect to pPROM without ACA. Follistatin mRNA expression did not differ between the groups. In placenta, nodal mRNA showed the same trend of activin A, while cripto was down-regulated in pPROM with ACA than other groups. Nodal and cripto were not expressed by fetal membranes.
The study shows the involvement of activin A pathway in pPROM with ACA. Further studies will focus on its role in placental immune functions.
研究与急性绒毛膜羊膜炎（ACA）相关的早产胎膜早破（pPROM）胎盘/胎膜中激活素 A 和相关分子的调节。
从自发性早产（PTD）、无 ACA 的 pPROM 和有 ACA 的 pPROM 妇女中获得组织。通过 RT-PCR 测量激活素 A、卵泡抑素、 nodal 和 cripto mRNA。
pPROM 组织中的激活素 A mRNA 在存在或不存在 HCA 的情况下均高于 PTD，并且在有 ACA 的 pPROM 中高于无 ACA 的 pPROM。各组间卵泡抑素 mRNA 表达无差异。在胎盘组织中，nodal mRNA 与激活素 A 呈相同趋势，而在有 ACA 的 pPROM 中 cripto 下调，低于其他组。nodal 和 cripto 均不由胎膜表达。
本研究表明激活素 A 途径参与了有 ACA 的 pPROM。进一步的研究将集中在其在胎盘免疫功能中的作用。
