Department of Forest Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju, South Korea.
J Environ Manage. 2012 Nov 30;111:142-9. doi: 10.1016/j.jenvman.2012.06.041. Epub 2012 Jul 28.
The treatment of effluents from textile industry with microorganisms, especially bacteria and fungi, has recently gained attention. The present study was conducted using white rot fungi Irpex lacteus, Trametes hirsuta, Trametes sp., and Lentinula edodes for the decolorization of reactive textile Levafix Blue E-RA granulate dye. I. lacteus resulted in the best decolorization and degradation of the dye within four days. Therefore, more detailed studies were carried out using I. lacteus. The decolorization was evaluated at various concentration, pH values, and temperatures. The activities of laccase, manganese peroxidase, and lignin peroxidase enzymes were estimated to reveal the roles of enzymes in decolorization. The colorless nature of the fungal cells revealed that decolorization occurred through degradation, and confirmed by analysis of the metabolites by UV-visible spectroscopy and High Performance Liquid Chromatography after decolorization. The metabolites were identified by Gas Chromatography-Mass Spectrometry, and functional group analysis was performed by Fourier Transform Infrared Spectroscopy. The degraded dye metabolites were assessed for phytotoxicity using Vigna radiata and Brassica juncea, which demonstrated nontoxic nature of the metabolites formed after degradation of dye.
利用微生物，特别是细菌和真菌，处理纺织工业废水最近受到了关注。本研究采用白腐真菌糙皮侧耳（Irpex lacteus）、粗毛栓菌（Trametes hirsuta）、栓菌（Trametes sp.）和香菇（Lentinula edodes）对活性染料莱凡法克斯蓝 E-RA 颗粒染料进行脱色。结果表明，糙皮侧耳在四天内对染料的脱色和降解效果最好。因此，我们使用糙皮侧耳进行了更详细的研究。在不同的浓度、pH 值和温度下评估了脱色效果。测定了漆酶、锰过氧化物酶和木质素过氧化物酶的活性，以揭示酶在脱色过程中的作用。真菌细胞无色表明脱色是通过降解发生的，通过在脱色后用紫外-可见光谱和高效液相色谱分析代谢物得到证实。通过气相色谱-质谱联用技术对代谢物进行了鉴定，并通过傅里叶变换红外光谱进行了官能团分析。利用豇豆和芥菜评估了降解染料代谢物的植物毒性，结果表明，染料降解后形成的代谢物无毒性。
