Department of Forest Science and Technology, Institute of Agricultural Science and Technology, Chonbuk National University, Jeonju, South Korea.
J Hazard Mater. 2011 Dec 30;198:198-205. doi: 10.1016/j.jhazmat.2011.10.030. Epub 2011 Oct 17.
The present study was conducted to evaluate the decolorization and degradation of the chromium metal complex dye Isolan Dark Blue 2SGL-01 by Irpex lacteus, a white rot lignolytic fungus. I. lacteus effectively decolorized the sulphonated reactive dye at a high concentration of 250 mg/l over a wide range of pH values of 5-9 and temperatures between 20 and 35°C. Complete (100%) decolorization occurred within 96h, and I. lacteus demonstrated resistance to the metallic dye. UV-vis spectroscopy, HPLC, GC-MS, and FT-IR analyses of the extracted metabolites confirmed that the decolorization process occurred due to degradation of the dye and not merely by adsorption. GC-MS analysis indicated the formation of 1(2H)-naphthalenone, 3,4-dihydro- and 2-naphthalenol as the main metabolite. ICP analysis demonstrated the removal of 13.49% chromium, and phytotoxicity studies using germinated seeds of Vigna radiata and Brassica juncea demonstrated the nontoxic nature of the metabolites formed during the degradation of Isolan Dark Blue 2SGL-01 dye.
本研究旨在评估白腐真菌糙皮侧耳（Irpex lacteus）对铬金属络合染料 Isolan 深兰 2SGL-01 的脱色和降解作用。在 20 至 35°C 的宽 pH 值范围（5-9）和高浓度（250mg/L）下，I. lacteus 可有效对经磺化的反应性染料进行脱色，在 96 小时内即可达到完全脱色（100%），且对金属染料具有抗性。提取代谢物的 UV-vis 光谱、HPLC、GC-MS 和 FT-IR 分析证实，该脱色过程是由于染料的降解所致，而非仅仅通过吸附作用。GC-MS 分析表明，主要代谢产物为 1(2H)-萘酮、3,4-二氢萘酚和 2-萘酚。ICP 分析表明铬的去除率为 13.49%，而利用豇豆和芥菜种子进行的发芽毒性研究表明，在 Isolan 深兰 2SGL-01 染料降解过程中形成的代谢物无毒性。
