Department of Blood Transfusion, Chinese PLA General Hospital, Beijing 100853, China.
Oxid Med Cell Longev. 2012;2012:209843. doi: 10.1155/2012/209843. Epub 2012 Jun 27.
Flavonoids are found in most parts of plants and have been shown to have multiple biological activities such as anticancer, anti-inflammation, antibacteria, antivirus, and immune-stimulation. Existing data showed that the total flavonoids of Astragalus (TFA) can provide biological system with resistance to injury and can possess antimutagenic, atherosclerotic inhibition, and other biological effects. This study investigated the effects of TFA and calycosin (a compound isolated from TFA), on apoptosis induction, and cell cycle of human erythroleukemia cell line K562 by an array of techniques, including proliferation (MTT), PI staining, Annexin V/PI double staining, and RT-PCR. The experimental data showed that TFA and calycosin could inhibit the proliferation of K562 cells. The 50% inhibiting concentrations of TFA and calycosin were 98.63 μg/mL and 130.32 μg/mL, respectively. However, TFA and calycosin could not induce apoptosis in K562 cells, but could increase the number of the cells in the G(0)/G(1) phase. The level of cyclin D1 mRNA in K562 cells decreased after the treatment with TFA and calycosin. This study provides new insights into the functional mechanism of total flavonoids of Astragalus and calycosin on human erythroleukemia cells.
类黄酮存在于植物的大部分部位，已被证明具有多种生物活性，如抗癌、抗炎、抗菌、抗病毒和免疫刺激。现有数据表明，黄芪总黄酮（TFA）可以为生物系统提供抗损伤能力，并具有抗突变、抑制动脉粥样硬化等生物效应。本研究采用增殖（MTT）、PI 染色、Annexin V/PI 双重染色和 RT-PCR 等一系列技术，研究了 TFA 和毛蕊异黄酮（从 TFA 中分离得到的一种化合物）对人红白血病细胞系 K562 细胞凋亡诱导和细胞周期的影响。实验数据表明，TFA 和毛蕊异黄酮能抑制 K562 细胞的增殖。TFA 和毛蕊异黄酮的 50%抑制浓度分别为 98.63μg/ml 和 130.32μg/ml。然而，TFA 和毛蕊异黄酮不能诱导 K562 细胞凋亡，但能增加 G(0)/G(1)期细胞数量。TFA 和毛蕊异黄酮处理后，K562 细胞中环细胞 D1mRNA 的水平降低。本研究为黄芪总黄酮和毛蕊异黄酮对人红白血病细胞的功能机制提供了新的见解。
