Key Laboratory of Mesoscopic Chemistry of MOE, College of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.
ACS Appl Mater Interfaces. 2012 Aug;4(8):4113-22. doi: 10.1021/am300878z. Epub 2012 Aug 9.
Mesoporous silica MCM-41 and SBA-15 were chosen to study the adsorption and release of bulky biomolecule heparin, in order to develop new heparin controlled delivery system and expand the application of mesoporous materials in life science. To explore how the structure of support such as pore size and surface state affects the accommodation and release of heparin, we used decane as swelling agent to enlarge pores of MCM-41, introduced amino groups for improving the biocompatibility of support, and controllably retained templates in the as-synthesized sample. The influence of modification on the structure of samples was investigated by XRD and N(2) adsorption-desorption, whereas their performance of adsorbing and releasing heparin was assessed with that of toluidine blue method. Both enlarged pore and organic modification significantly promoted the adsorption and prolonged the release of heparin in MCM-41, and the release was characterized with a three-stage release model. The mechanism of heparin release from mesoporous material was studied by fitting the release profiles to the theoretical equation. As expected, some mesoporous composites could release heparin in the long term with tuned dosage.
介孔硅材料 MCM-41 和 SBA-15 被选择用来研究大生物分子肝素的吸附和释放，以开发新的肝素控制释放体系并拓展介孔材料在生命科学中的应用。为了探索载体结构（如孔径和表面状态）如何影响肝素的容纳和释放，我们使用癸烷作为膨胀剂来扩大 MCM-41 的孔，引入氨基以提高载体的生物相容性，并在合成的样品中可控地保留模板。通过 XRD 和 N2 吸附-脱附研究了修饰对样品结构的影响，而用甲苯胺蓝法评估了它们吸附和释放肝素的性能。扩大孔径和有机修饰都显著促进了肝素在 MCM-41 中的吸附并延长了肝素的释放，释放具有三阶段释放模型的特点。通过将释放曲线拟合到理论方程来研究肝素从介孔材料中的释放机制。正如预期的那样，一些介孔复合材料可以通过调整剂量来实现肝素的长期释放。
