促性腺类固醇处理对大鼠弓状核 Kiss1mRNA 表达的影响在出生后发育过程中。
The effects of gonadal steroid manipulation on the expression of Kiss1 mRNA in rat arcuate nucleus during postnatal development.
机构信息
Department of Anatomy and Neurobiology, Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan.
出版信息
J Physiol Sci. 2012 Nov;62(6):453-60. doi: 10.1007/s12576-012-0222-y. Epub 2012 Aug 1.
Abstract
Kisspeptins, encoded by Kiss1 gene, play pivotal roles in the regulation of reproduction. Recently, several studies reported a sex difference in Kiss1 expression in the arcuate nucleus (ARC) during the neonatal period. In this study, we investigated the effect of gonadal steroid manipulation on the sex difference in Kiss1 expression in ARC of rats. At neonatal and prepubertal stages, females had a greater number of Kiss1 neurons than the males. Gonadectomy at those stages resulted in significant increases in the Kiss1 neuron number and the sex differences disappeared. We also confirmed the expression of estrogen receptor α in kisspeptin neurons in neonates. Altogether, our results indicate that ARC Kiss1 expression is negatively regulated by gonadal steroids from early postnatal stages, and that the sex difference in ARC Kiss1 expression is attributed to the difference in circulating gonadal steroid levels. We also found that neonatal estrogenization inhibits Kiss1 expression and impairs negative feedback system.
摘要
Kisspeptins 由 Kiss1 基因编码，在生殖调控中发挥着关键作用。最近，有几项研究报道了在新生儿期弓状核（ARC）中 Kiss1 表达存在性别差异。在这项研究中，我们研究了性腺类固醇处理对大鼠 ARC 中 Kiss1 表达性别差异的影响。在新生儿期和青春期前阶段，女性的 Kiss1 神经元数量多于男性。在这些阶段进行性腺切除术会导致 Kiss1 神经元数量显著增加，性别差异消失。我们还在新生儿中证实了雌激素受体 α 在 kisspeptin 神经元中的表达。总之，我们的研究结果表明，ARC Kiss1 表达受出生后早期性腺类固醇的负向调节，ARC Kiss1 表达的性别差异归因于循环性腺类固醇水平的差异。我们还发现，新生儿期雌激素化抑制 Kiss1 表达并损害负反馈系统。
相似文献
1
The effects of gonadal steroid manipulation on the expression of Kiss1 mRNA in rat arcuate nucleus during postnatal development.促性腺类固醇处理对大鼠弓状核 Kiss1mRNA 表达的影响在出生后发育过程中。
J Physiol Sci. 2012 Nov;62(6):453-60. doi: 10.1007/s12576-012-0222-y. Epub 2012 Aug 1.
2
Sex differences in the regulation of Kiss1/NKB neurons in juvenile mice: implications for the timing of puberty.青春期启动调控中，幼年小鼠 Kiss1/NKB 神经元的性别差异及其意义。
Am J Physiol Endocrinol Metab. 2009 Nov;297(5):E1212-21. doi: 10.1152/ajpendo.00461.2009. Epub 2009 Sep 15.
3
Sexually dimorphic expression of hypothalamic estrogen receptors α and β and Kiss1 in neonatal male and female rats.新生雄性和雌性大鼠下丘脑雌激素受体 α 和 β 及 Kiss1 的性别二态性表达。
J Comp Neurol. 2011 Oct 15;519(15):2954-77. doi: 10.1002/cne.22648.
4
Increased neurokinin B (Tac2) expression in the mouse arcuate nucleus is an early marker of pubertal onset with differential sensitivity to sex steroid-negative feedback than Kiss1.弓状核中神经激肽 B（Tac2）表达增加是青春期启动的早期标志物，其对性激素负反馈的敏感性与 Kiss1 不同。
Endocrinology. 2012 Oct;153(10):4883-93. doi: 10.1210/en.2012-1529. Epub 2012 Aug 14.
5
Developmental expression patterns of gonadal hormone receptors in arcuate kisspeptin and GABA neurons of the postnatal female mouse.产后雌性小鼠弓状核吻素神经元和γ-氨基丁酸能神经元中性腺激素受体的发育表达模式
J Neuroendocrinol. 2025 Jan;37(1):e13477. doi: 10.1111/jne.13477. Epub 2024 Nov 28.
6
Dissecting the KNDy hypothesis: KNDy neuron-derived kisspeptins are dispensable for puberty but essential for preserved female fertility and gonadotropin pulsatility.解析 KNDy 假说：KNDy 神经元衍生的 kisspeptins 对于青春期是可有可无的，但对于保持雌性生育力和促性腺激素脉冲性是必不可少的。
Metabolism. 2023 Jul;144:155556. doi: 10.1016/j.metabol.2023.155556. Epub 2023 Apr 29.
7
Disparate changes in kisspeptin and neurokinin B expression in the arcuate nucleus after sex steroid manipulation reveal differential regulation of the two KNDy peptides in rats.性类固醇操纵后弓状核中 kisspeptin 和神经激肽 B 表达的不同变化揭示了大鼠中两种 KNDy 肽的差异调节。
Endocrinology. 2014 Oct;155(10):3945-55. doi: 10.1210/en.2014-1200. Epub 2014 Jul 22.
8
Gene expression pattern of Kisspeptin and RFamide-related peptide (Rfrp) in the male Damaraland mole-rat hypothalamus.男性达马拉兰鼹形田鼠下丘脑 Kisspeptin 和 RFamide 相关肽 (Rfrp) 的基因表达模式。
J Chem Neuroanat. 2021 Dec;118:102039. doi: 10.1016/j.jchemneu.2021.102039. Epub 2021 Oct 14.
9
Should We Make More Bone or Not, As Told by Kisspeptin Neurons in the Arcuate Nucleus.究竟该造骨还是不造骨，视弓状核的 kisspeptin 神经元所言。
Semin Reprod Med. 2019 May;37(3):147-150. doi: 10.1055/s-0039-3400238. Epub 2019 Dec 23.
10
ERα Signaling in GHRH/Kiss1 Dual-Phenotype Neurons Plays Sex-Specific Roles in Growth and Puberty.生长激素释放激素/ kiss1 双表型神经元中的 ERα 信号在生长和青春期中发挥性别特异性作用。
J Neurosci. 2020 Dec 2;40(49):9455-9466. doi: 10.1523/JNEUROSCI.2069-20.2020. Epub 2020 Nov 6.
引用本文的文献
1
Comprehensive chemoanatomical mapping, and the gonadal regulation, of seven kisspeptin neuronal populations in the mouse brain.小鼠脑中七个 kisspeptin 神经元群体的综合化学解剖图谱及性腺调节
J Neuroendocrinol. 2025 May;37(5):e70019. doi: 10.1111/jne.70019. Epub 2025 Mar 18.
2
Kisspeptin fiber and receptor distribution analysis suggests its potential role in central sensorial processing and behavioral state control.亲吻素纤维和受体分布分析表明其在中枢感觉处理和行为状态控制中的潜在作用。
J Neuroendocrinol. 2025 May;37(5):e70007. doi: 10.1111/jne.70007. Epub 2025 Mar 10.
3
Single neonatal estrogen implant sterilizes female animals by decreasing hypothalamic KISS1 expression.单个新生雌性激素植入物通过减少下丘脑 KISS1 表达来使雌性动物绝育。
Sci Rep. 2023 Jun 14;13(1):9627. doi: 10.1038/s41598-023-36727-8.
4
Long-term effects of prenatal undernutrition on female rat hypothalamic KNDy neurons.产前营养不足对雌性大鼠下丘脑KNDy神经元的长期影响。
Endocr Connect. 2022 Dec 15;12(1). doi: 10.1530/EC-22-0307. Print 2023 Jan 1.
5
Neurochemical Characterization of Neurons Expressing Estrogen Receptor β in the Hypothalamic Nuclei of Rats Using in Situ Hybridization and Immunofluorescence.利用原位杂交和免疫荧光技术研究大鼠下丘脑核内表达雌激素受体β的神经元的神经化学特征。
Int J Mol Sci. 2019 Dec 23;21(1):115. doi: 10.3390/ijms21010115.
6
The Role of Kisspeptin in Female Reproduction.亲吻素在女性生殖中的作用。
Int J Endocrinol Metab. 2017 Apr 22;15(3):e44337. doi: 10.5812/ijem.44337. eCollection 2017 Jul.
7
Hypothalamic KISS1 expression, gonadotrophin-releasing hormone and neurotransmitter innervation vary with stress and sensitivity in macaques.下丘脑KISS1的表达、促性腺激素释放激素和神经递质支配在猕猴中随应激和敏感性而变化。
J Neuroendocrinol. 2014 May;26(5):267-81. doi: 10.1111/jne.12146.
8
Lack of functional GABAB receptors alters Kiss1 , Gnrh1 and Gad1 mRNA expression in the medial basal hypothalamus at postnatal day 4.功能性 GABAB 受体缺乏会改变新生后第 4 天内侧基底下丘脑的 Kiss1、Gnrh1 和 Gad1 mRNA 的表达。
Neuroendocrinology. 2013;98(3):212-23. doi: 10.1159/000355631. Epub 2013 Nov 6.
9
Development and Aging of the Kisspeptin-GPR54 System in the Mammalian Brain: What are the Impacts on Female Reproductive Function?哺乳动物脑内 kisspeptin-GPR54 系统的发育和衰老：对女性生殖功能有何影响？
Front Endocrinol (Lausanne). 2013 Mar 28;4:22. doi: 10.3389/fendo.2013.00022. eCollection 2013.
本文引用的文献
1
Sexually dimorphic testosterone secretion in prenatal and neonatal mice is independent of kisspeptin-Kiss1r and GnRH signaling.雄性和雌性小鼠在产前和新生儿期的性二态性睾丸素分泌独立于 kisspeptin-Kiss1r 和 GnRH 信号。
Endocrinology. 2012 Feb;153(2):782-93. doi: 10.1210/en.2011-1838. Epub 2011 Dec 27.
2
Sexually dimorphic expression of hypothalamic estrogen receptors α and β and Kiss1 in neonatal male and female rats.新生雄性和雌性大鼠下丘脑雌激素受体 α 和 β 及 Kiss1 的性别二态性表达。
J Comp Neurol. 2011 Oct 15;519(15):2954-77. doi: 10.1002/cne.22648.
3
BAX-dependent and BAX-independent regulation of Kiss1 neuron development in mice.BAX 依赖性和非依赖性调节小鼠 Kiss1 神经元发育。
Endocrinology. 2010 Dec;151(12):5807-17. doi: 10.1210/en.2010-0783. Epub 2010 Oct 6.
4
Developmental changes in the expression of kisspeptin mRNA in rat hypothalamus.大鼠下丘脑 kisspeptin mRNA 表达的发育变化。
J Mol Neurosci. 2011 Feb;43(2):138-45. doi: 10.1007/s12031-010-9430-1. Epub 2010 Jul 28.
5
Sex differences in the regulation of Kiss1/NKB neurons in juvenile mice: implications for the timing of puberty.青春期启动调控中，幼年小鼠 Kiss1/NKB 神经元的性别差异及其意义。
Am J Physiol Endocrinol Metab. 2009 Nov;297(5):E1212-21. doi: 10.1152/ajpendo.00461.2009. Epub 2009 Sep 15.
6
Significance of neonatal testicular sex steroids to defeminize anteroventral periventricular kisspeptin neurons and the GnRH/LH surge system in male rats.新生儿睾丸性激素对雄性大鼠前脑室下吻肽神经元和 GnRH/LH 峰系统去女性化的意义。
Biol Reprod. 2009 Dec;81(6):1216-25. doi: 10.1095/biolreprod.109.078311. Epub 2009 Aug 14.
7
Sex differences and the roles of sex steroids in apoptosis of sexually dimorphic nuclei of the preoptic area in postnatal rats.出生后大鼠视前区性二态核凋亡中的性别差异及性类固醇的作用。
J Neuroendocrinol. 2009 Mar;21(4):370-6. doi: 10.1111/j.1365-2826.2009.01855.x.
8
Persistent impairment of hypothalamic KiSS-1 system after exposures to estrogenic compounds at critical periods of brain sex differentiation.在大脑性别分化的关键时期暴露于雌激素化合物后，下丘脑KiSS-1系统持续受损。
Endocrinology. 2009 May;150(5):2359-67. doi: 10.1210/en.2008-0580. Epub 2008 Dec 23.
9
Kisspeptin immunoreactive neurons in the equine hypothalamus Interactions with GnRH neuronal system.马下丘脑内的促性腺激素释放激素神经元 kisspeptin 免疫反应性神经元与促性腺激素释放激素神经元系统的相互作用。
J Chem Neuroanat. 2008 Dec;36(3-4):131-7. doi: 10.1016/j.jchemneu.2008.07.008. Epub 2008 Aug 7.
10
Structural interactions between kisspeptin and GnRH neurons in the mediobasal hypothalamus of the male rhesus monkey (Macaca mulatta) as revealed by double immunofluorescence and confocal microscopy.双重免疫荧光和共聚焦显微镜揭示的雄性恒河猴（猕猴）中脑基底下丘脑内 kisspeptin 与促性腺激素释放激素（GnRH）神经元之间的结构相互作用
Endocrinology. 2008 Sep;149(9):4387-95. doi: 10.1210/en.2008-0438. Epub 2008 May 29.
Zotero 插件