Department of Pneumonology and Allergy, Medical University of Lodz, Poland.
Arch Med Sci. 2012 Jul 4;8(3):515-20. doi: 10.5114/aoms.2012.28639.
Oxidative stress is a non-specific feature of airway inflammation in asthmatics. 8-Isoprostane (8-IP), a prostaglandin-F(2α) isomer, is a relatively new marker of oxidative stress and may be measured in exhaled breath condensate (EBC) of patients with asthma. This research study aimed to evaluate the usefulness of EBC 8-IP as a marker of severity and control of severe adult asthma.
Twenty-seven severe, never-smoking asthmatics were studied. According to positive or negative reversibility testing, this group was subdivided into reversible and irreversible asthma groups. All participants were observed for 8 weeks during which they completed daily diary observations including day and night symptoms, number of awakenings, peak expiratory flow (PEF) variability, daily rescue medication usage and oral steroids consumption. They attended the clinic 3 times and on these occasions spirometry assessments, EBC collection and asthma control tests (ACT) were done. Two control groups were included: 11 healthy never-smokers and 16 newly diagnosed and never-treated, non-smoking mild asthmatics.
There were no statistically significant differences between severe asthma and healthy control or never-treated asthma groups in concentrations of EBC 8-IP (median and interquartile range: 4.67; 2.50-27.92 vs. 6.93; 2.5-12.98 vs. 3.80; 2.50-10.73, respectively). No correlations were found between EBC 8-IP and asthma control parameters, such as ACT results, night and day symptoms, consumption of rescue medication, percentage of days free of oral steroids, PEF diurnal variation, lung function test results, forced expiratory volume in the 1 s reversibility, and markers of systemic inflammation.
Our study results suggest that EBC 8-IP measurements are not useful for asthma monitoring.
氧化应激是哮喘患者气道炎症的非特异性特征。8-异前列腺素（8-IP），一种前列腺素 F（2α）异构体，是氧化应激的一个相对较新的标志物，可在哮喘患者的呼出气冷凝液（EBC）中测量。本研究旨在评估 EBC 8-IP 作为严重成人哮喘严重程度和控制的标志物的有用性。
研究了 27 名从未吸烟的严重哮喘患者。根据阳性或阴性可逆性测试，将该组分为可逆性和不可逆性哮喘组。所有参与者在 8 周内接受观察，在此期间他们完成了日常日记观察，包括白天和夜间症状、觉醒次数、呼气峰流速（PEF）变异性、每日急救药物使用和口服类固醇消耗。他们就诊 3 次，在此期间进行了肺功能评估、EBC 收集和哮喘控制测试（ACT）。包括了 2 个对照组：11 名从未吸烟的健康者和 16 名新诊断且从未治疗的非吸烟轻度哮喘者。
严重哮喘组与健康对照组或未经治疗的哮喘组之间，EBC 8-IP 的浓度（中位数和四分位距：4.67；2.50-27.92 与 6.93；2.5-12.98 与 3.80；2.50-10.73）没有统计学差异。EBC 8-IP 与哮喘控制参数之间没有相关性，如 ACT 结果、白天和夜间症状、急救药物使用、无口服类固醇天数百分比、PEF 日变异率、肺功能测试结果、1 秒用力呼气量的可逆性、和全身炎症标志物。
我们的研究结果表明，EBC 8-IP 测量对哮喘监测没有帮助。
