Zanconato Stefania, Carraro Silvia, Corradi Massimo, Alinovi Rossella, Pasquale Maria Francesca, Piacentini Giorgio, Zacchello Franco, Baraldi Eugenio
Department of Pediatrics, University of Padova, Padova, Italy.
J Allergy Clin Immunol. 2004 Feb;113(2):257-63. doi: 10.1016/j.jaci.2003.10.046.
Cysteinyl-leukotrienes (cys-LTs) and 8-isoprostane are biomarkers of airway inflammation and oxidative stress.
The aim of this study was to evaluate cys-LT and 8-isoprostane levels in exhaled breath condensate (EBC) of children with different degrees of asthma severity.
EBC was collected from 14 steroid-naive children with mild persistent asthma, 13 children with stable mild- to-moderate persistent asthma treated with inhaled corticosteroids (ICS), 9 ICS-treated children with unstable asthma, and 19 healthy children.
In the three groups of asthmatic children, EBC concentrations of cys-LTs and 8-isoprostane were significantly higher than in control children (steroid-naive asthmatic children: cys-LTs median, 10.8 pg/mL, P <.001, 8-isoprostane, 16.2 pg/mL, P <.001; ICS-treated stable asthmatic children: cys-LTs, 12.7 pg/mL, P <.001, 8-isoprostane, 18.1 pg/mL, P <.001; children with unstable asthma: cys-LTs, 106.0 pg/mL, P <.01, 8-isoprostane, 29.7 pg/mL, P <.01; control children: cys-LTs, 4.3 pg/mL, 8-isoprostane, 3.5 pg/mL). Cys-LT levels were higher in children with unstable asthma than in the other two asthmatic groups (P <.05). FE(NO) levels were significantly higher in steroid-naive and in children with unstable asthma compared with ICS-treated children with stable asthma (P <.01).
Our study shows that EBC cys-LTs and 8-isoprostane concentrations are higher in asthmatic children than in healthy control children, with scattered values in patients with unstable asthma. These findings suggest that EBC eicosanoid measurement may have useful clinical implications for investigating phenotype differences among asthmatic patients.
半胱氨酰白三烯（cys-LTs）和8-异前列腺素是气道炎症和氧化应激的生物标志物。
本研究旨在评估不同哮喘严重程度儿童呼出气冷凝液（EBC）中的cys-LT和8-异前列腺素水平。
收集了14例未使用过类固醇的轻度持续性哮喘儿童、13例接受吸入性糖皮质激素（ICS）治疗的稳定的轻度至中度持续性哮喘儿童、9例接受ICS治疗的不稳定哮喘儿童以及19例健康儿童的EBC。
在三组哮喘儿童中，EBC中cys-LTs和8-异前列腺素的浓度显著高于对照儿童（未使用过类固醇的哮喘儿童：cys-LTs中位数为10.8 pg/mL，P<.001，8-异前列腺素为16.2 pg/mL，P<.001；接受ICS治疗的稳定哮喘儿童：cys-LTs为12.7 pg/mL，P<.001，8-异前列腺素为18.1 pg/mL，P<.001；不稳定哮喘儿童：cys-LTs为106.0 pg/mL，P<.01，8-异前列腺素为29.7 pg/mL，P<.01；对照儿童：cys-LTs为4.3 pg/mL，8-异前列腺素为3.5 pg/mL）。不稳定哮喘儿童的cys-LT水平高于其他两组哮喘儿童（P<.05）。与接受ICS治疗的稳定哮喘儿童相比，未使用过类固醇的儿童和不稳定哮喘儿童的FE(NO)水平显著更高（P<.01）。
我们的研究表明，哮喘儿童EBC中cys-LTs和8-异前列腺素的浓度高于健康对照儿童，不稳定哮喘患者的值分散。这些发现表明，EBC类二十烷酸测量可能对研究哮喘患者的表型差异具有有用的临床意义。
