Sakaue Hirotaka, Dan Risako, Shimizu Megumi, Kazama Haruko
Aerospace Research and Development Directorate, Japan Aerospace Exploration Agency, Chofu, Tokyo 182-8522, Japan.
Rev Sci Instrum. 2012 Jul;83(7):076106. doi: 10.1063/1.4737875.
Microscopic in vivo pH imaging system is developed that can capture the luminescent- and color-imaging. The former gives a quantitative measurement of a pH distribution in vivo. The latter captures the structural information that can be overlaid to the pH distribution for correlating the structure of a specimen and its pH distribution. By using a digital color camera, a luminescent image as well as a color image is obtained. The system uses HPTS (8-hydroxypyrene-1,3,6-trisulfonate) as a luminescent pH indicator for the luminescent imaging. Filter units are mounted in the microscope, which extract two luminescent images for using the excitation-ratio method. A ratio of the two images is converted to a pH distribution through a priori pH calibration. An application of the system to epidermal cells of Lactuca Sativa L is shown.
开发了一种微观体内pH成像系统，该系统可以进行发光成像和彩色成像。前者可对体内pH分布进行定量测量。后者捕获的结构信息可以叠加到pH分布上，以便将标本的结构与其pH分布相关联。通过使用数字彩色相机，可以获得发光图像和彩色图像。该系统使用HPTS（8-羟基芘-1,3,6-三磺酸盐）作为发光成像的发光pH指示剂。滤光单元安装在显微镜中，通过激发比法提取两张发光图像。通过先验pH校准，将这两张图像的比值转换为pH分布。展示了该系统在生菜表皮细胞上的应用。
