Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
Med Mycol. 2013 Feb;51(2):208-13. doi: 10.3109/13693786.2012.701338. Epub 2012 Aug 2.
The karyotype of Microsporum canis was analyzed by contoured-clamped homogeneous electric field (CHEF) gel electrophoresis. Four chromosomal bands that correspond to five chromosomes ranging from 3.0-6.2 Mb were identified, adding the total genome size to approximately 24.9 Mb. To confirm the number of chromosomes in M. canis, the number of telomeres was assessed by using a telomeric probe (TTAGGG)(4) in Southern blot analyses of digested genomic DNA. Treatment of M. canis DNA with Bal31 exonuclease revealed progressive shortening of the DNA fragments positive for the (TTAGGG)(4) sequence, supporting location of repeats at the chromosome ends. These results can aid in improving the understanding of the genetic characterization of M. canis and the molecular epidemiology of dermatophytoses caused by this fungus.
采用限制性内切酶电泳技术对犬小孢子菌的核型进行分析。鉴定出了 4 条染色体带，对应于 5 条染色体，大小从 3.0-6.2Mb，这将总基因组大小增加到约 24.9Mb。为了确认犬小孢子菌的染色体数量，通过 Southern blot 分析消化后的基因组 DNA 中的端粒探针（TTAGGG）（4）来评估端粒的数量。用 Bal31 核酸外切酶处理犬小孢子菌 DNA 后，（TTAGGG）（4）序列阳性的 DNA 片段逐渐缩短，这支持了重复序列位于染色体末端的位置。这些结果有助于提高对犬小孢子菌遗传特征和这种真菌引起的皮肤真菌病分子流行病学的理解。
