State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, 650091, P.R. China.
Sci Rep. 2017 Mar 30;7:45456. doi: 10.1038/srep45456.
Subtilases belong to a superfamily of serine proteases which are ubiquitous in fungi and are suspected to have developed distinct functional properties to help fungi adapt to different ecological niches. In this study, we conducted a large-scale phylogenomic survey of subtilase protease genes in 83 whole genome sequenced fungal species in order to identify the evolutionary patterns and subsequent functional divergences of different subtilase families among the main lineages of the fungal kingdom. Our comparative genomic analyses of the subtilase superfamily indicated that extensive gene duplications, losses and functional diversifications have occurred in fungi, and that the four families of subtilase enzymes in fungi, including proteinase K-like, Pyrolisin, kexin and S53, have distinct evolutionary histories which may have facilitated the adaptation of fungi to a broad array of life strategies. Our study provides new insights into the evolution of the subtilase superfamily in fungi and expands our understanding of the evolution of fungi with different lifestyles.
枯草溶菌素属于丝氨酸蛋白酶超家族，在真菌中普遍存在，被怀疑具有独特的功能特性，以帮助真菌适应不同的生态位。在这项研究中，我们对 83 个全基因组测序的真菌物种中的枯草溶菌素蛋白酶基因进行了大规模的系统基因组调查，以鉴定真菌王国主要谱系中不同枯草溶菌素家族的进化模式和随后的功能分化。我们对枯草溶菌素超家族的比较基因组分析表明，真菌中发生了广泛的基因复制、缺失和功能多样化，真菌中的四种枯草溶菌素酶家族，包括蛋白酶 K 样、Pyrolisin、kexin 和 S53，具有不同的进化历史，这可能有助于真菌适应广泛的生活策略。我们的研究为真菌中枯草溶菌素超家族的进化提供了新的见解，并扩展了我们对不同生活方式的真菌进化的理解。
