Hightower R C, Callahan T M, Upchurch R G
Agricultural Research Service, North Carolina State University, Raleigh 27695-7615, USA.
Curr Genet. 1995 Feb;27(3):290-2. doi: 10.1007/BF00326163.
Classical genetic analyses are not possible with the phytopathogenic fungus Cercospora kikuchii since no sexual stage has been identified. To facilitate gene mapping and to develop an understanding of the genome organization of C. kikuchii, an electrophoretic karyotype has been obtained using contour-clamped homogeneous electric field gel electrophoresis (CHEF). Eight chromosomes, two of which migrate as a doublet, have been separated into seven bands ranging from 2.0 to 5.5 Mb. Using this determination of chromosome number and size, the total genome size of C. kikuchii is estimated to be 28.4 Mb. In addition, genes encoding tubulin, ribosomal DNA, and four previously isolated light-enhanced cDNAs from C. kikuchii were assigned to chromosomes by Southern-hybridization analysis of CHEF blots.
由于尚未鉴定出致病真菌菊池尾孢菌的有性阶段，因此无法对其进行经典的遗传分析。为了便于基因定位并深入了解菊池尾孢菌的基因组组织，已使用轮廓夹恒定电场凝胶电泳（CHEF）获得了其电泳核型。八条染色体（其中两条以双峰形式迁移）已被分离为七条带，大小从2.0到5.5 Mb不等。根据这种对染色体数量和大小的测定，估计菊池尾孢菌的基因组总大小为28.4 Mb。此外，通过对CHEF印迹进行Southern杂交分析，将编码微管蛋白、核糖体DNA的基因以及先前从菊池尾孢菌中分离出的四个光增强cDNA定位到了染色体上。
