Passenger strand miRNA miR-31* regulates the phenotypes of oral cancer cells by targeting RhoA.

OBJECTIVES

MicroRNAs (miRNAs) are endogenous small non-coding RNAs that negatively regular target gene expression by RNA interference. The processing of the pre-miRNA hairpin generates a miRNA duplex, which consists of a miRNA (guide strand) and a miRNA() (passenger strand). miR-31 is an oncogenic miRNA and is up-regulated in oral squamous cell carcinoma (OSCC). miR-31() shows a high level of conservation across species and, based on this, this study hypothesized that miR-31(*) is a functional miRNA.

MATERIALS AND METHODS

The expression of miR-31 and miR-31* in OSCC tissues and oral cells were analyzed. Functional studies were performed on OSCC cells.

RESULTS

miR-31() is up-regulated in OSCC tissues, but its expression is less abundant than miR-31. miR-31() decreases the proliferation and migration of both SAS and Fadu cells. Furthermore, miR-31() targets the 3'UTR of RhoA and is able to down-regulate RhoA expression. Knockdown of RhoA expression is known to decrease the proliferation and migration of OSCC cells. However, up-regulation of both miR-31 and miR-31() by delivery of pre-mir-31 does still enhance OSCC oncogenicity.

CONCLUSION

miR-31() is a functional miRNA involving in regulating RhoA, and the activity of miR-31()'s activity seems to counteract the functions of miR-31 during OSCC tumorigenesis.