Kumar Selvaraj Rajesh, Hsu Ya-Hui, Vi Truong Thi Tuong, Pang Jong-Hwei Su, Lee Yao-Chang, Hsieh Chia-Hsun, Lue Shingjiang Jessie
Department of Chemical and Materials Engineering, Chang Gung University, Wenhua 1st Road, Guishan, Taoyuan 333, Taiwan.
Graduate Institute of Clinical Medical Sciences, Chang Gung University, Wenhua 1st Road, Guishan, Taoyuan 333, Taiwan.
Materials (Basel). 2021 Mar 13;14(6):1396. doi: 10.3390/ma14061396.
The objectives of this work aim to investigate the interaction and cytotoxicity between nanometric graphene oxide (GO) and nasopharyngeal carcinoma cells (NPC-BM1), and possible application in photon therapy. GO nanosheets were obtained in the size range of 100-200 nm, with a negative surface charge. This nanometric GO exhibited a limited (<10%) cytotoxicity effect and no significant dimensional change on NPC-BM1 cells in the tested GO concentration range (0.1-10 µg·mL). However, the secondary protein structure was modified in the GO-treated NPC-BM1 cells, as determined through synchrotron radiation-based Fourier transform infrared microspectroscopy (SR-FTIRM) mapping. To further study the cellular response of GO-treated NPC-BM1 cancer cells at low GO concentration (0.1 µg·mL), photon radiation was applied with increasing doses, ranging from 2 to 8 Gy. The low radiation energy (<5 Gy) did not cause significant cell mortality (5-7%). Increasing the radiation energy to 6-8 Gy accelerated cell apoptosis rate, especially in the GO-treated NPC-BM1 cells (27%). This necrosis may be due to GO-induced conformational changes in protein and DNA/RNA, resulting in cell vulnerability under photon radiation. The findings of the present work demonstrate the potential biological applicability of nanometric GO in different areas, such as targeted drug delivery, cellular imaging, and radiotherapy, etc.
这项工作的目标旨在研究纳米氧化石墨烯（GO）与鼻咽癌细胞（NPC-BM1）之间的相互作用和细胞毒性，以及在光子治疗中的可能应用。获得的GO纳米片尺寸范围为100 - 200 nm，表面带负电荷。在测试的GO浓度范围（0.1 - 10 µg·mL）内，这种纳米级GO对NPC-BM1细胞表现出有限的（<10%）细胞毒性作用，且细胞尺寸无显著变化。然而，通过基于同步辐射的傅里叶变换红外显微光谱（SR-FTIRM）映射测定，GO处理的NPC-BM1细胞中的二级蛋白质结构发生了改变。为了进一步研究低浓度GO（0.1 µg·mL）处理的NPC-BM1癌细胞的细胞反应，施加了剂量不断增加的光子辐射，范围从2到8 Gy。低辐射能量（<5 Gy）未导致显著的细胞死亡率（5 - 7%）。将辐射能量增加到6 - 8 Gy会加速细胞凋亡率，尤其是在GO处理的NPC-BM1细胞中（27%）。这种坏死可能是由于GO诱导的蛋白质和DNA/RNA构象变化，导致细胞在光子辐射下变得脆弱。本工作的研究结果证明了纳米级GO在不同领域，如靶向药物递送、细胞成像和放射治疗等方面潜在的生物学适用性。
