López Omaira Y, López Myriam C, Corredor Vladimir, Echeverri M Clara, Pinilla Análida E
Departamento de Salud Pública, Facultad de Medicina, Universidad Nacional de Colombia, Colombia.
Rev Med Chil. 2012 Apr;140(4):476-83. doi: 10.4067/S0034-98872012000400008.
Entamoeba histolytica and Entamoeba dispar are morphologically identical. However, the former is highly pathogenic and the latter is not.
To differentiate Entamoeba histolytica from Entamoeba dispar through ELISA and PCR techniques in Colombian isolates from feces.
Descriptive study of Colombian fecal samples from 53 males and 47 women, that were positive for the complex E. histolytica/E. dispar on light microscopy. Positive samples were cultured on Robinson medium to isolate trophozoites. The presence of specific Gal/ GalNAc-lectin was determined by ELISA and polymerase chain reaction in genomic DNA, using the combination of three nucleotides that recognize a variable region of 16S small subunit ribosomal RNA, generating a 166 base pair (bp) product for E. histolytica and 752 pb product for E. dispar.
After verification, only eight of the 100 samples were positive for the complex E. histolytica/E. dispar and were cultivated. Isolates were obtained in six cultures, one corresponded to E. histolytica and six to E. dispar.
The presence of E. histolytica/E. dispar complex was largely overestimated with light microscopy. In the few samples where isolates were obtained, the technique described differentiated between both strains.
溶组织内阿米巴和迪斯帕内阿米巴在形态上相同。然而，前者具有高度致病性，而后者则无致病性。
通过酶联免疫吸附测定（ELISA）和聚合酶链反应（PCR）技术，对哥伦比亚粪便分离株中的溶组织内阿米巴和迪斯帕内阿米巴进行鉴别。
对53名男性和47名女性的哥伦比亚粪便样本进行描述性研究，这些样本在光学显微镜下对溶组织内阿米巴/迪斯帕内阿米巴复合体呈阳性。将阳性样本接种于罗宾逊培养基上培养以分离滋养体。采用ELISA法和聚合酶链反应检测基因组DNA中特异性半乳糖/ N - 乙酰半乳糖胺凝集素的存在，使用识别16S小亚基核糖体RNA可变区的三个核苷酸组合，溶组织内阿米巴产生166碱基对（bp）的产物，迪斯帕内阿米巴产生752 pb的产物。
经核实，100份样本中只有8份对溶组织内阿米巴/迪斯帕内阿米巴复合体呈阳性并进行了培养。在6次培养中获得了分离株，其中1株为溶组织内阿米巴，6株为迪斯帕内阿米巴。
光学显微镜对溶组织内阿米巴/迪斯帕内阿米巴复合体的存在情况存在很大程度的高估。在少数获得分离株的样本中，所描述的技术能够区分这两种菌株。
