Benedek Tibor, Szentgyörgyi Flóra, Gergócs Veronika, Menashe Ofir, Gonzalez Perla Abigail Figueroa, Probst Alexander J, Kriszt Balázs, Táncsics András
Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Gödöllő, Páter K. u. 1, 2100, Hungary.
Institute for Soil Sciences, Budapest, Herman Ottó út 15, 1022, Hungary.
AMB Express. 2021 Sep 6;11(1):126. doi: 10.1186/s13568-021-01289-3.
Here, we report and discuss the applicability of Variovorax paradoxus strain BFB1_13 in the bioremediation of BTEX contaminated sites. Strain BFB1_13 was capable of degrading all the six BTEX-compounds under both aerobic (O conc. 8 mg l) and micro-aerobic/oxygen-limited (O conc. 0.5 mg l) conditions using either individual (8 mg‧l) or a mixture of compounds (~ 1.3 mg‧l of each BTEX compound). The BTEX biodegradation capability of SBP-encapsulated cultures (SBP-Small Bioreactor Platform) was also assessed. The fastest degradation rate was observed in the case of aerobic benzene biodegradation (8 mg l per 90 h). Complete biodegradation of other BTEX occurred after at least 168 h of incubation, irrespective of the oxygenation and encapsulation. No statistically significant difference was observed between aerobic and microaerobic BTEX biodegradation. Genes involved in BTEX biodegradation were annotated and degradation pathways were predicted based on whole-genome shotgun sequencing and metabolic analysis. We conclude that V. paradoxus strain BFB1_13 could be used for the development of reactive biobarriers for the containment and in situ decontamination of BTEX contaminated groundwater plumes. Our results suggest that V. paradoxus strain BFB1_13-alone or in co-culture with other BTEX degrading bacterial isolates-can be a new and efficient commercial bioremediation agent for BTEX contaminated sites.
在此，我们报告并讨论了奇异贪噬菌菌株BFB1_13在生物修复BTEX污染场地中的适用性。菌株BFB1_13能够在有氧（氧气浓度8 mg·l）和微需氧/限氧（氧气浓度0.5 mg·l）条件下，使用单一化合物（8 mg·l）或化合物混合物（每种BTEX化合物约1.3 mg·l）降解所有六种BTEX化合物。还评估了SBP封装培养物（SBP-小型生物反应器平台）的BTEX生物降解能力。在有氧苯生物降解（每90小时8 mg·l）的情况下观察到最快的降解速率。无论氧化和封装情况如何，其他BTEX在孵育至少168小时后完全生物降解。有氧和微需氧BTEX生物降解之间未观察到统计学上的显著差异。基于全基因组鸟枪法测序和代谢分析，对参与BTEX生物降解的基因进行了注释并预测了降解途径。我们得出结论，奇异贪噬菌菌株BFB1_13可用于开发反应性生物屏障，以遏制和原位净化受BTEX污染的地下水羽流。我们的结果表明，奇异贪噬菌菌株BFB1_13单独使用或与其他降解BTEX的细菌分离物共培养，都可以成为受BTEX污染场地新型高效的商业生物修复剂。
