Intensive Care Unit, Guangdong Province Hospital of Chinese Medicine, Guangzhou 510120, China.
Chin J Integr Med. 2012 Aug;18(8):610-5. doi: 10.1007/s11655-012-1175-y. Epub 2012 Aug 2.
To study the effects of tetramethylpyrazine (TMP) on cardiac function and mortality rate in septic rats.
Fifty male Sprague-Dawley rats were randomized into a sham-operation group (sham group, n=10), normal saline group (NS group, n=20), and TMP group (n=20). The rats in the NS and TMP groups underwent cecal ligation and puncture (CLP) to induce sepsis. Rats in the NS group were injected with NS (10 mL/kg) immediately after CLP and 6 h after CLP. Rats in the TMP group were injected with TMP (10 mg/kg) at the same time points. Twenty-four hours after modeling, the mortality rates were observed in each group. Cardiac function and serum concentration of tumor necrosis factor α (TNF-α) were also tested. The correlation between TNF-α and the ejection fraction (EF) was observed. Left ventricle specimens were reserved for histomorphologic study.
Compared with the sham group, the NS and TMP groups had decreased EF values and increased mortality rates and serum TNF-α levels (P <0.05). The TMP group had a comparatively lower mortality rate and TNF-α level and a higher EF value compared with the NS group (P <0.05). Histomorphology indicated that myocardial inflammation in the TMP group was mild compared with that in the NS group. There was a negative correlation between TNF-α level and EF value (r=-0.583,P=0.000).
TMP could reduce the mortality rate of septic rats and had certain protective effects on cardiac function.
研究川芎嗪（TMP）对脓毒症大鼠心功能和死亡率的影响。
将 50 只雄性 Sprague-Dawley 大鼠随机分为假手术组（sham 组，n=10）、生理盐水组（NS 组，n=20）和 TMP 组（n=20）。NS 组和 TMP 组大鼠行盲肠结扎穿孔术（CLP）以诱导脓毒症。NS 组大鼠在 CLP 后即刻和 6 h 时给予 NS（10 mL/kg）注射。TMP 组大鼠在相同时间点给予 TMP（10 mg/kg）注射。建模 24 h 后，观察各组大鼠的死亡率。还检测了心功能和血清肿瘤坏死因子-α（TNF-α）浓度。观察 TNF-α与射血分数（EF）的相关性。保留左心室标本进行组织形态学研究。
与 sham 组相比，NS 组和 TMP 组 EF 值降低，死亡率和血清 TNF-α水平升高（P<0.05）。与 NS 组相比，TMP 组死亡率和 TNF-α水平较低，EF 值较高（P<0.05）。组织形态学显示，TMP 组心肌炎症较 NS 组轻。TNF-α水平与 EF 值呈负相关（r=-0.583，P=0.000）。
TMP 可降低脓毒症大鼠的死亡率，并对心功能具有一定的保护作用。
