Nutritional Epidemiology Branch, Division of Cancer Epidemiology and Genetics, National Cancer Institute, 6120 Executive Boulevard, Room 3028, Bethesda, Maryland 20892-7246, USA.
J Clin Endocrinol Metab. 2012 Oct;97(10):3724-33. doi: 10.1210/jc.2012-1732. Epub 2012 Aug 1.
The objective of the study was to evaluate in premenopausal women the relationships of physically active and sedentary behaviors reported for adulthood and adolescence with a comprehensive profile of estrogen metabolism.
Fifteen estrogens and estrogen metabolites (jointly termed EM) were measured using liquid chromatography-tandem mass spectrometry in luteal phase urines from 603 premenopausal women in the Nurses' Health Study II. Geometric means of individual EM, metabolic pathway groups, and pathway ratios were examined by level of exposure after adjustment for age, body mass index, alcohol intake, menstrual cycle length, and sample collection timing.
High overall physical activity in adulthood (42+ metabolic equivalent h/wk vs. <3 metabolic equivalent h/wk) was associated with a 15% lower level of urinary estradiol (Ptrend=0.03) and 15% lower level of 16-hydroxylation pathway EM (Ptrend=0.03). Levels of 2- and 4-hydroxylation pathway EM did not differ significantly by physical activity. High overall activity was also positively associated with four ratios: 2-pathway EM to parent estrogens (Ptrend=0.05), 2-pathway catechols to parent estrogens (Ptrend=0.03), 2-pathway catechols to methylated 2-pathway catechols (Ptrend<0.01), and 2-hydroxyestrone to 16α-hydroxyestrone (Ptrend=0.01). Similar patterns of association were noted for walking and vigorous physical activity, but there was little evidence of associations with sedentary behaviors or activity during adolescence.
High levels of physical activity were associated with lower levels of parent estrogens and 16-hydroxylation pathway EM and preferential metabolism to 2-pathway catechols. The results of our analysis, the largest, most comprehensive examination of physical activity and estrogen metabolism to date, may be useful in future studies investigating the etiology of diseases linked to both physical activity and endogenous estrogen.
本研究旨在评估成年期和青春期活跃和久坐行为与雌激素代谢综合特征的关系，研究对象为绝经前女性。
采用液相色谱-串联质谱法检测 603 名绝经前妇女黄体期尿液中的 15 种雌激素及其代谢物（统称 EM）。在调整年龄、体重指数、酒精摄入量、月经周期长度和样本采集时间后，按暴露水平评估个体 EM、代谢途径组和途径比值的几何均数。
成年期总体体力活动水平较高（42+代谢当量/周与 <3 代谢当量/周），与尿雌二醇水平降低 15%（趋势 P=0.03）和 16-羟化途径 EM 降低 15%（趋势 P=0.03）相关。2-和 4-羟化途径 EM 水平与体力活动无显著差异。总体活动水平较高与四个比值呈正相关：2-途径 EM 与母雌激素（趋势 P=0.05）、2-途径儿茶酚与母雌激素（趋势 P=0.03）、2-途径儿茶酚与甲基化 2-途径儿茶酚（趋势 P<0.01）和 2-羟雌酮与 16α-羟雌酮（趋势 P=0.01）。步行和剧烈体力活动也存在类似的关联模式，但几乎没有证据表明与久坐行为或青春期活动有关。
高水平的体力活动与母雌激素和 16-羟化途径 EM 水平降低以及优先代谢为 2-途径儿茶酚有关。我们的分析结果是目前对体力活动和雌激素代谢进行的最大、最全面的研究，可能对未来研究与体力活动和内源性雌激素相关的疾病病因学具有重要意义。
