Institute of Insect Science, Zhejiang University, Hangzhou 310058, PR China.
Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, PR China.
J Gen Virol. 2012 Nov;93(Pt 11):2480-2489. doi: 10.1099/vir.0.043836-0. Epub 2012 Aug 1.
A baculovirus, named BomaNPV S2, was isolated from a diseased larva of the wild silkworm, Bombyx mandarina. Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both Bombyx mori nucleopolyhedrosis virus (BmNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. It could replicate in cells of B. mori (Bm5 and BmN), Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn-5B1-4). However, occlusion-derived virions of BomaNPV S2 in B. mori cells contained only a single nucleocapsid, whereas they contained multiple nucleocapsids in Tn-5B1-4 cells. The complete genome sequence of BomaNPV S2, including predicted ORFs, was determined and compared with the genome sequence of its close relatives. The comparison results showed that most of the BomaNPV S2 genome sequence was shared with BmNPV (BmNPV T3) or BomaNPV S1, but several regions seemed more similar to regions of AcMNPV. This observation might explain why BomaNPV S2 covers the host ranges of BmNPV and AcMNPV. Further recombinant virus infection experiments demonstrated that GP64 plays an important role in BomaNPV S2 host-range determination.
一种杆状病毒，命名为 BomaNPV S2，从野生家蚕的患病幼虫中分离出来。值得注意的是，BomaNPV S2 具有一个独特的特征，即其宿主范围涵盖了家蚕核型多角体病毒（BmNPV）和美洲棉铃虫多角体病毒（AcMNPV）在培养细胞中的宿主范围。它可以在 B. mori（Bm5 和 BmN）、草地贪夜蛾（Sf9）和斜纹夜蛾（Tn-5B1-4）的细胞中复制。然而，BomaNPV S2 在 B. mori 细胞中形成的包埋病毒粒子只含有一个核衣壳，而在 Tn-5B1-4 细胞中则含有多个核衣壳。确定了 BomaNPV S2 的完整基因组序列，包括预测的 ORFs，并与近亲的基因组序列进行了比较。比较结果表明，BomaNPV S2 的大部分基因组序列与 BmNPV（BmNPV T3）或 BomaNPV S1 共享，但有几个区域似乎与 AcMNPV 的区域更相似。这一观察结果可能解释了为什么 BomaNPV S2 涵盖了 BmNPV 和 AcMNPV 的宿主范围。进一步的重组病毒感染实验表明，GP64 在 BomaNPV S2 宿主范围决定中起着重要作用。
