Smirnova T D, Danilenko D M, Eropkin M Iu, Deeva E G, Kiselev O I
Antibiot Khimioter. 2011;56(11-12):11-6.
The influence of antivirals, such as rimantadine, ribavirine and triazavirine on influenza virus replication in human cell cultures was evaluated. All the antivirals inhibited viral nucleoprotein NP synthesis. The strongest effect was shown for ribavirine in lung carcinoma A-549 cells and endothelial ECV-304 cells. Hoechst-33258 staining revealed induction of apoptosis in all the cell lines. Rimantadine and ribavirine inhibited virus-induced apoptosis while ribavirine enhanced it. The effect was registered in monolayer cell cultures as well as in suspension cell cultures. The influence of the antiviral drugs on the virus-induced cell proliferation in the suspension cell cultures is also described.
评估了金刚乙胺、利巴韦林和三氮唑核苷等抗病毒药物对流感病毒在人细胞培养物中复制的影响。所有抗病毒药物均抑制病毒核蛋白NP的合成。利巴韦林在肺癌A - 549细胞和内皮ECV - 304细胞中显示出最强的效果。Hoechst - 33258染色显示所有细胞系均有凋亡诱导。金刚乙胺和利巴韦林抑制病毒诱导的凋亡，而利巴韦林则增强凋亡。这种作用在单层细胞培养物和悬浮细胞培养物中均有记录。还描述了抗病毒药物对悬浮细胞培养物中病毒诱导的细胞增殖的影响。
