Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong, China.
PLoS One. 2012;7(7):e41645. doi: 10.1371/journal.pone.0041645. Epub 2012 Jul 31.
Avian pathogenic Escherichia coli (APEC) leads to economic losses in poultry production and is also a threat to human health. The goal of this study was to characterize the chicken spleen transcriptome and to identify candidate genes for response and resistance to APEC infection using Solexa sequencing. We obtained 14422935, 14104324, and 14954692 Solexa read pairs for non-challenged (NC), challenged-mild pathology (MD), and challenged-severe pathology (SV), respectively. A total of 148197 contigs and 98461 unigenes were assembled, of which 134949 contigs and 91890 unigenes match the chicken genome. In total, 12272 annotated unigenes take part in biological processes (11664), cellular components (11927), and molecular functions (11963). Summing three specific contrasts, 13650 significantly differentially expressed unigenes were found in NC Vs. MD (6844), NC Vs. SV (7764), and MD Vs. SV (2320). Some unigenes (e.g. CD148, CD45 and LCK) were involved in crucial pathways, such as the T cell receptor (TCR) signaling pathway and microbial metabolism in diverse environments. This study facilitates understanding of the genetic architecture of the chicken spleen transcriptome, and has identified candidate genes for host response to APEC infection.
禽致病性大肠杆菌（APEC）导致家禽生产的经济损失，也是对人类健康的威胁。本研究的目的是用 Solexa 测序来描述鸡脾脏转录组的特征，并鉴定对 APEC 感染的反应和抗性的候选基因。我们分别获得了 14422935、14104324 和 14954692 个 Solexa 读对用于非攻毒（NC）、攻毒轻度病变（MD）和攻毒重度病变（SV）。共组装了 148197 个 contigs 和 98461 个 unigenes，其中 134949 个 contigs 和 91890 个 unigenes与鸡基因组匹配。共有 12272 个注释的 unigenes参与了生物过程（11664）、细胞成分（11927）和分子功能（11963）。对三个特定对照进行总结，在 NC 与 MD（6844）、NC 与 SV（7764）和 MD 与 SV（2320）之间发现了 13650 个显著差异表达的 unigenes。一些 unigenes（如 CD148、CD45 和 LCK）参与了关键途径，如 T 细胞受体（TCR）信号途径和微生物代谢在不同环境中的作用。本研究有助于理解鸡脾脏转录组的遗传结构，并鉴定了宿主对 APEC 感染的反应的候选基因。
