• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

宿主与病原体在禽类全血模型中的动态相互作用。

Dynamic Interplay of Host and Pathogens in an Avian Whole-Blood Model.

机构信息

Research Group Microbial Immunology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institut, Jena, Germany.

Research Group Applied Systems Biology, Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institut, Jena, Germany.

出版信息

Front Immunol. 2020 Mar 31;11:500. doi: 10.3389/fimmu.2020.00500. eCollection 2020.

DOI:10.3389/fimmu.2020.00500
PMID:32296424
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7136455/
Abstract

Microbial survival in blood is an essential step toward the development of disseminated diseases and blood stream infections. For poultry, however, little is known about the interactions of host cells and pathogens in blood. We established an chicken whole-blood infection assay as a tool to analyze interactions between host cells and three model pathogens, , and . Following a systems biology approach, we complemented the experimental measurements with functional and quantitative immune characteristics by virtual infection modeling. All three pathogens were killed in whole blood, but each to a different extent and with different kinetics. Monocytes, and to a lesser extent heterophils, associated with pathogens. Both association with host cells and transcriptional activation of genes encoding immune-associated functions differed depending on both the pathogen and the genetic background of the chickens. Our results provide first insights into quantitative interactions of three model pathogens with different immune cell populations in avian blood, demonstrating a broad spectrum of different characteristics during the immune response that depends on the pathogen and the chicken line.

摘要

微生物在血液中的存活是其向传播性疾病和血流感染发展的关键步骤。然而，对于家禽而言，人们对宿主细胞与血液中病原体之间的相互作用知之甚少。我们建立了一个鸡全血感染模型，以此来分析宿主细胞与三种模式病原体（ 、 、 ）之间的相互作用。采用系统生物学方法，我们通过虚拟感染建模，将实验测量与功能和定量免疫特征相结合。三种病原体均在全血中被杀死，但每种病原体的杀伤程度和动力学均有所不同。单核细胞和程度较轻的异嗜性白细胞与病原体相关。与宿主细胞的关联以及免疫相关功能基因的转录激活，均取决于病原体和鸡的遗传背景。我们的研究结果首次深入了解了三种模式病原体与禽类血液中不同免疫细胞群体的定量相互作用，表明在免疫反应过程中存在广泛的不同特征，这些特征取决于病原体和鸡系。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/b15ce254d6e3/fimmu-11-00500-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/f706335ce147/fimmu-11-00500-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/3895b0fdeb11/fimmu-11-00500-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/3da0ff6d7f5d/fimmu-11-00500-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/fee971c803a1/fimmu-11-00500-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/bc2af8b1be56/fimmu-11-00500-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/a2a74cf45224/fimmu-11-00500-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/896468fd63a1/fimmu-11-00500-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/e663cdc14581/fimmu-11-00500-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/5a86c48db851/fimmu-11-00500-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/b15ce254d6e3/fimmu-11-00500-g0010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/f706335ce147/fimmu-11-00500-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/3895b0fdeb11/fimmu-11-00500-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/3da0ff6d7f5d/fimmu-11-00500-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/fee971c803a1/fimmu-11-00500-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/bc2af8b1be56/fimmu-11-00500-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/a2a74cf45224/fimmu-11-00500-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/896468fd63a1/fimmu-11-00500-g0007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/e663cdc14581/fimmu-11-00500-g0008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/5a86c48db851/fimmu-11-00500-g0009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/69e2/7136455/b15ce254d6e3/fimmu-11-00500-g0010.jpg

相似文献

1
Dynamic Interplay of Host and Pathogens in an Avian Whole-Blood Model.宿主与病原体在禽类全血模型中的动态相互作用。
Front Immunol. 2020 Mar 31;11:500. doi: 10.3389/fimmu.2020.00500. eCollection 2020.
2
Chicken endothelial cells are highly responsive to viral innate immune stimuli and are susceptible to infections with various avian pathogens.鸡内皮细胞对病毒固有免疫刺激高度敏感，且易受多种禽病原体感染。
Avian Pathol. 2019 Apr;48(2):121-134. doi: 10.1080/03079457.2018.1556386. Epub 2019 Jan 10.
3
Highly multiplexed quantitative PCR-based platform for evaluation of chicken immune responses.高通量定量 PCR 平台评估鸡的免疫反应。
PLoS One. 2019 Dec 3;14(12):e0225658. doi: 10.1371/journal.pone.0225658. eCollection 2019.
4
Precision cut lung slices: a novel versatile tool to examine host-pathogen interaction in the chicken lung.精密切割肺切片：一种新型多功能工具，用于研究鸡肺中的宿主-病原体相互作用。
Vet Res. 2020 Jan 10;51(1):2. doi: 10.1186/s13567-019-0733-0.
5
Avian lipocalin expression in chickens following Escherichia coli infection and inhibition of avian pathogenic Escherichia coli growth by Ex-FABP.大肠杆菌感染后鸡体内禽脂联素的表达以及Ex-FABP对禽致病性大肠杆菌生长的抑制作用
Vet Immunol Immunopathol. 2013 Mar 15;152(1-2):156-67. doi: 10.1016/j.vetimm.2012.09.018. Epub 2012 Sep 26.
6
Avian Pathogenic (APEC) Strain-Dependent Immunomodulation of Respiratory Granulocytes and Mononuclear Phagocytes in -Reporter Transgenic Chickens.禽致病性（APEC）菌株对-报告基因转基因鸡呼吸性粒细胞和单核吞噬细胞的免疫调节作用。
Front Immunol. 2020 Jan 10;10:3055. doi: 10.3389/fimmu.2019.03055. eCollection 2019.
7
Recent human-to-poultry host jump, adaptation, and pandemic spread of Staphylococcus aureus.金黄色葡萄球菌近期从人类宿主向禽类宿主的跨宿主传播、适应性变化及大流行传播。
Proc Natl Acad Sci U S A. 2009 Nov 17;106(46):19545-50. doi: 10.1073/pnas.0909285106. Epub 2009 Nov 2.
8
Immunogenomic approaches to study host immunity to enteric pathogens.研究宿主对肠道病原体免疫的免疫基因组学方法。
Poult Sci. 2007 Jul;86(7):1491-500. doi: 10.1093/ps/86.7.1491.
9
Immunostimulatory properties of Toll-like receptor ligands in chickens.鸡体内Toll样受体配体的免疫刺激特性
Vet Immunol Immunopathol. 2013 Apr 15;152(3-4):191-9. doi: 10.1016/j.vetimm.2012.10.013. Epub 2012 Dec 13.
10
Predictive Virtual Infection Modeling of Fungal Immune Evasion in Human Whole Blood.预测性虚拟真菌感染模型在人全血中的真菌免疫逃逸研究。
Front Immunol. 2018 Mar 21;9:560. doi: 10.3389/fimmu.2018.00560. eCollection 2018.

引用本文的文献

1
Complex-mediated evasion: modeling defense against antimicrobial peptides with application to human-pathogenic fungus Candida albicans.复合物介导的逃避：对抗微生物肽防御机制的建模及其在人类致病真菌白色念珠菌中的应用
NPJ Syst Biol Appl. 2025 Jul 22;11(1):81. doi: 10.1038/s41540-025-00559-1.
2
Compensatory mechanisms in γδ T cell-deficient chickens following infection.γδ T细胞缺陷型鸡感染后的代偿机制。
Front Immunol. 2025 May 14;16:1576766. doi: 10.3389/fimmu.2025.1576766. eCollection 2025.
3
Spatiotemporal modeling quantifies cellular contributions to uptake of Aspergillus fumigatus in the human lung.

本文引用的文献

1
Rapid whole blood assay using flow cytometry for measuring phagocytic activity of chicken leukocytes.使用流式细胞术进行全血快速检测以测量鸡白细胞的吞噬活性。
Vet Immunol Immunopathol. 2019 Jan;207:53-61. doi: 10.1016/j.vetimm.2018.11.014. Epub 2018 Nov 28.
2
Selective breeding for high natural antibody level increases resistance to avian pathogenic Escherichia coli (APEC) in chickens.对高天然抗体水平进行选择性育种可提高鸡对禽致病性大肠杆菌（APEC）的抵抗力。
Dev Comp Immunol. 2019 Apr;93:45-57. doi: 10.1016/j.dci.2018.12.007. Epub 2018 Dec 20.
3
Inflammasomes in livestock and wildlife: Insights into the intersection of pathogens and natural host species.
时空建模量化了细胞对人类肺部烟曲霉摄取的贡献。
Commun Biol. 2024 Dec 4;7(1):1615. doi: 10.1038/s42003-024-07302-2.
4
Surrogate infection model predicts optimal alveolar macrophage number for clearance of Aspergillus fumigatus infections.替代感染模型预测清除烟曲霉感染的最佳肺泡巨噬细胞数量。
NPJ Syst Biol Appl. 2023 Apr 10;9(1):12. doi: 10.1038/s41540-023-00272-x.
5
Differential gene expression reveals host factors for viral shedding variation in mallards () infected with low-pathogenic avian influenza virus.差异基因表达揭示了低致病性禽流感病毒感染的野鸭（）病毒脱落变异的宿主因素。
J Gen Virol. 2022 Mar;103(3). doi: 10.1099/jgv.0.001724.
6
Interaction of Salmonella Gallinarum and Salmonella Enteritidis with peripheral leucocytes of hens with different laying performance.鸡不同产蛋性能外周白细胞与鸡白痢沙门氏菌和肠炎沙门氏菌的相互作用。
Vet Res. 2021 Sep 25;52(1):123. doi: 10.1186/s13567-021-00994-y.
7
Comparative assessment of immune evasion mechanisms in human whole-blood infection assays by a systems biology approach.系统生物学方法比较人全血感染模型中的免疫逃逸机制。
PLoS One. 2021 Apr 1;16(4):e0249372. doi: 10.1371/journal.pone.0249372. eCollection 2021.
8
Significant Differences in Host-Pathogen Interactions Between Murine and Human Whole Blood.宿主-病原体相互作用在鼠类和人类全血中存在显著差异。
Front Immunol. 2021 Jan 15;11:565869. doi: 10.3389/fimmu.2020.565869. eCollection 2020.
9
Hybrid Agent-Based Modeling of Infection to Quantitatively Investigate the Role of Pores of Kohn in Human Alveoli.基于混合智能体模型的感染研究，以定量探究科恩孔在人类肺泡中的作用。
Front Microbiol. 2020 Aug 12;11:1951. doi: 10.3389/fmicb.2020.01951. eCollection 2020.
家畜和野生动物中的炎性小体：对病原体与天然宿主物种交叉点的见解。
Vet Immunol Immunopathol. 2018 Jul;201:49-56. doi: 10.1016/j.vetimm.2018.05.008. Epub 2018 May 21.
4
Avian pathogenic Escherichia coli-induced activation of chicken macrophage HD11 cells.禽致病性大肠杆菌诱导鸡巨噬细胞HD11细胞的激活。
Dev Comp Immunol. 2018 Oct;87:75-83. doi: 10.1016/j.dci.2018.05.019. Epub 2018 May 23.
5
Quantitative Simulations Predict Treatment Strategies Against Fungal Infections in Virtual Neutropenic Patients.定量模拟预测针对虚拟中性粒细胞减少症患者真菌感染的治疗策略。
Front Immunol. 2018 Apr 4;9:667. doi: 10.3389/fimmu.2018.00667. eCollection 2018.
6
Predictive Virtual Infection Modeling of Fungal Immune Evasion in Human Whole Blood.预测性虚拟真菌感染模型在人全血中的真菌免疫逃逸研究。
Front Immunol. 2018 Mar 21;9:560. doi: 10.3389/fimmu.2018.00560. eCollection 2018.
7
Phagosomal Neutralization by the Fungal Pathogen Candida albicans Induces Macrophage Pyroptosis.真菌病原体白色念珠菌对吞噬体的中和作用诱导巨噬细胞焦亡。
Infect Immun. 2017 Jan 26;85(2). doi: 10.1128/IAI.00832-16. Print 2017 Feb.
8
Poultry: the most common food in outbreaks with known pathogens, United States, 1998-2012.家禽：1998 - 2012年美国已知病原体引发疫情中最常见的食物。
Epidemiol Infect. 2017 Jan;145(2):316-325. doi: 10.1017/S0950268816002375. Epub 2016 Oct 26.
9
Transcriptome Profile of the Chicken Thrombocyte: New Implications as an Advanced Immune Effector Cell.鸡血小板的转录组概况：作为高级免疫效应细胞的新意义。
PLoS One. 2016 Oct 6;11(10):e0163890. doi: 10.1371/journal.pone.0163890. eCollection 2016.
10
Non-specific activation of CD8α-characterised γδ T cells in PBL cultures of different chicken lines.不同鸡系外周血淋巴细胞培养物中CD8α特征性γδ T细胞的非特异性激活。
Vet Immunol Immunopathol. 2016 Oct 15;179:1-7. doi: 10.1016/j.vetimm.2016.07.008. Epub 2016 Jul 15.