Biosensor AN Ltd., pr. Akademika Semenova 1, 142432 Chernogolovka, Moscow Region, Russia.
Biochemistry (Mosc). 2012 Aug;77(8):843-56. doi: 10.1134/S0006297912080056.
This review considers quinone-dependent alcohol dehydrogenases and FAD-dependent alcohol oxidases, enzymes that are present in numerous methylotrophic eu- and prokaryotes and significantly differ in their primary and quaternary structure. The cofactors of the enzymes are bound to the protein polypeptide chain through ionic and hydrophobic interactions. Microorganisms containing these enzymes are described. Methods for purification of the enzymes, their physicochemical properties, and spatial structures are considered. The supposed mechanism of action and practical application of these enzymes as well as their producers are discussed.
这篇综述探讨了醌依赖性醇脱氢酶和 FAD 依赖性醇氧化酶，这些酶存在于许多甲基营养型真核生物和原核生物中，在一级和四级结构上有显著差异。酶的辅因子通过离子和疏水相互作用结合到蛋白质多肽链上。描述了含有这些酶的微生物。考虑了这些酶的纯化方法、物理化学性质和空间结构。讨论了这些酶的作用机制、实际应用及其产生菌。
