Bui Lam Dinh, Tsai Po-Chun, Nguyen Tuan Ngoc, Chen Chih-Chen, Huang Shir-Ly
Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei, 112304, Taiwan.
Group of Applied Research in Advanced Materials for Sustainable Development, Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, 700000, Vietnam.
Appl Microbiol Biotechnol. 2025 Jun 4;109(1):137. doi: 10.1007/s00253-025-13463-9.
Pseudomonas nitroreducens ATCC PTA-6168 (also named TX1) efficiently grows on non-ionic surfactants as the sole source of carbon and energy aerobically. The global gene expression and bacterial stress responses during degradation remain further investigated. This study compared the growth, viability, and transcriptomic profiles of strain TX1 during the log phase when grown on minimal salts basal medium supplemented with 0.5% octylphenol polyethoxylates (OPEO, commercial name Triton X-100) vs. in 0.5% succinate. Differentially expressed genes were identified, with 219 upregulated and 22 downregulated. Gene ontology analysis revealed upregulation of oxidoreductase activity (53%), electrotransfer activity (11%), heme/FAD/cofactor binding (11/19/27%), and membrane (42%)-related functions. In ethanol oxidation, adh18 and adh19 (for two pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenases), aldh1 and aldh2 (for two aldehyde dehydrogenases), c (for cytochrome c), and pqqABCDEF (for PQQ biosynthesis proteins) and aceA (for isocitrate lyase) and aceB (for malate synthase) acting in the glyoxylate cycle were upregulated. In addition to the above genes, the expression level of the representative genes from fatty acid degradation and chemotaxis was verified by RT-qPCR. The global gene expression of P. nitroreducens TX1 provides candidate genes involved in the bacteria grown on nonionic surfactants. KEY POINTS: • Growth characteristics of Pseudomonas nitroreducens ATCC PTA-6168 on surfactants • Differentially expressed genes related to growth on surfactants were identified • The ethanol oxidation system was upregulated when grown on surfactants.
嗜氮假单胞菌ATCC PTA - 6168（也称为TX1）能够在非离子表面活性剂作为唯一碳源和能源的条件下进行高效好氧生长。在降解过程中的全局基因表达和细菌应激反应仍有待进一步研究。本研究比较了菌株TX1在补充0.5%辛基酚聚乙氧基化物（OPEO，商品名吐温X - 100）的基本盐培养基上对数期生长时与在0.5%琥珀酸盐中生长时的生长、活力和转录组谱。鉴定出差异表达基因，其中219个上调，22个下调。基因本体分析显示氧化还原酶活性（53%）、电子转移活性（11%）、血红素/FAD/辅因子结合（11/19/27%）以及与膜（42%）相关的功能上调。在乙醇氧化过程中，adh18和adh19（两种依赖吡咯喹啉醌（PQQ）的乙醇脱氢酶）、aldh1和aldh2（两种醛脱氢酶）、c（细胞色素c）、pqqABCDEF（PQQ生物合成蛋白）以及在乙醛酸循环中起作用的aceA（异柠檬酸裂解酶）和aceB（苹果酸合酶）均上调。除上述基因外，还通过RT - qPCR验证了脂肪酸降解和趋化性代表性基因的表达水平。嗜氮假单胞菌TX1的全局基因表达提供了参与该细菌在非离子表面活性剂上生长的候选基因。要点：•嗜氮假单胞菌ATCC PTA - 6168在表面活性剂上的生长特性•鉴定了与在表面活性剂上生长相关的差异表达基因•在表面活性剂上生长时乙醇氧化系统上调。
